铽离子增敏环丙沙星研究及其应用  

Enhancement of terbium ion to ciprofloxacin and its application

作　　者：戚雪勇[1] 何华[2] 顾飞[2] 余江河[3] 

机构地区：[1]江苏大学药学院,江苏镇江212001 [2]中国药科大学分析化学教研室,江苏南京210009 [3]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《中国药学杂志》2005年第19期1500-1503,共4页Chinese Pharmaceutical Journal

基　　金：江苏省自然科学基金(KB2003080)

摘　　要：目的建立胶囊和血样中环丙沙星的含量测定方法。方法在醋酸-醋酸钠缓冲液(pH6.0)中,铽(Tb^(3+))能与环丙沙星(ciprofloxacin)形成络合物,该络合物吸收紫外光后,能发射铽的特征荧光。λ_(ex)/λ_(em)=325.0/545.0,狭缝均为5nm。结果线性范围1.0×10^(-8)～1.0×10^(-6)mol·L^(-1)。回归方程为F=8.26×10~8c+18.5(r=0.9999)(c:mol·L^(-1));检出限为3.5μg·L^(-1)。结论该法用于血清和胶囊中环丙沙星的含量测定,简单、快速、灵敏,结果令人满意。OBJECTIVE To establish a method for measuring the concentration of ciprofloxacin（CIP） in capsule and blood.METHODS In the NaAc-HAc buffer solution（pH 6.0） ,Tb^3＋- CIP chelate system was formed. The fluorescence emission of Tb^3＋ was detected after the chelate compound absorbed ultraviolet light. The λex and λem were 325.0 nm and 545.0 nm, respectively, and the slit widthes was both of 5 nm. RESULTS Under the optimal experimental conditions, the linear range of the determination was 1.0×10^-8-1.0×10^-6 mol·L^-1, the regression equation was F=8.26×10^8c＋ 18.5（r=0.999 9）（c：mol·L^-1）, the detection limit was 3.5μg·L^-1.CONCLUSION The method is simple,rapid and sensitive.

关 键 词：铽 环丙沙星 增敏 含量测定 

分 类 号：R927[医药卫生—药学]