花生黄曲霉侵染抗性的AFLP标记  

Identification of AFLP Markers for Resistance to Seed Infection by Aspergillus flavus in Peanut(Arachis hypogaea L.)

作　　者：雷永[1] 廖伯寿[1] 王圣玉[1] 李栋[1] 姜慧芳[1] 

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062

出　　处：《作物学报》2005年第10期1349-1353,共5页Acta Agronomica Sinica

基　　金：国家自然科学基金(30170561);国家高技术研究发展计划(2002AA207010)

摘　　要：本研究利用抗、感黄曲霉侵染的花生品种为亲本配制杂交组合“中花5号×J11”,以其F2分离群体为研究材料,采用AFLP技术和BSA分析方法,获得了与花生黄曲霉菌侵染抗性连锁的2个分子标记,标记与抗性间的遗传距离分别为8.8 cM和6.6 cM;利用获得的分子标记对抗、感黄曲霉的花生种质资源进行了分子鉴定,试验结果表明,分子标记与抗性鉴定结果具有较高的一致性,证实了两标记应用于研究群体之外的育种潜力。该抗侵染分子标记的建立为开展花生抗黄曲霉侵染辅助选择育种提供了有效的筛选技术。Aflatoxin contamination is an important constraint to peanut industry worldwide. Genetic improvement for host resistance in peanut to fungal infection and aflatoxin production has been the best approach for integrated management of the problem. However, the progress in peanut breeding for resistance to aflatoxin is limited due to various reasons, among which, lack of cost-effective method for resistance identification in breeding materials or segregating progenies has been encountered in most breeding programs. Hence there is a need to develop a rapid and reliable screening method for selecting Aspergillus flavus infection resistance in peanut. The present study reported two DNA markers closely linked with resistance to A. flavus infection using AFLP technique based on bulked segregant pools derived from F2 progeny of Zhonghua 5 × J11. J11 is resistant to seed infection by Aspergillus flavus, while Zhonghua 5 is susceptible, two DNA markers linked with resistance to A. flavus infection were identified. The two specific fragments linked with seed infection resistance were, one about 440 bp which was named as marker E45/M53-440, and the other was about 520 bp, named as marker E44/M53-520, the former marker linkage distance to the resistant gene was 6.6 cM, while the latter was 8.8 cM. Twenty peanut genotypos with resistance to infection of A. flavus and seven susceptible genotypos were used to verify the reliability of the resistance markers, and high correlation between the molecular markers and the resistance result. The result shows that the potential of the markers which can be used in other resistant peanut genotypes to seed infection by A.flavus.

关 键 词：花生 黄曲霉菌 AFLP标记 标记辅助选择 

分 类 号：S565.2[农业科学—作物学]