转反义PLD_γ基因小麦的分子检测及农艺性状分析  

Molecular Detection of Transformed Wheat with Anti-PLD_γ Gene and Its Agronomic Characters

作　　者：王瑞霞[1] 高庆荣[1] 崔德才[2] 刘正斌[1] 乔晓琳[1] 李洪利 

机构地区：[1]山东农业大学农学院 [2]山东农业大学生命科学学院 [3]五岳泰山种业有限公司,山东泰安271018

出　　处：《作物学报》2005年第10期1354-1358,共5页Acta Agronomica Sinica

基　　金：国家"863"计划(2002AA207004);山东省自然科学基金(Y2002D08)资助

摘　　要：利用改良的穗茎注射法将反义PLD，基因导人普通小麦兰考906，经特异PCR扩增和PCR-Southem杂交技术筛选出转化体，并分析研究了转化株系与受体性状的差异。结果表明：（1）3个材料扩增出与阳性质粒大小相同的400bp的目的片段，说明该基因已整合到小麦的基因组中，获得了转基因小麦植株。（2）转基因株系在农艺性状上与受体存在显著差异，主要表现为株高、千粒重、单株穗数增加。变化范围分别为79．2．83．8cm、50．53～52．98g、5．30．8．86穗，分别比对照提高13．4cm、6．94g3．82穗；而穗长、小穗数、穗粒数减少。（3）转基因株系的成熟期明显比受体提早4．8d；转化株系早代和后代中均存在完全的雄性不育现象。Antisense phospholipase Dγ （PLDγ） gene was introduced into wheat cuhivar LK906 mediated by improved earstem injecting of Agrobacterium. The transformants were screened by PCR and PCR-Southern technique and the variation between transformation lines and receptor was analyzed. The results indicated that（ 1 ）400 bp special fragment to the plasmid was amplified in three plants （03039, 03048, 03050）, which showed that anti-PLDγ gene was integrated into wheat genomes, thus the transgenic wheats of anti-PLDγ gene were obtained. （2） There were significant differences between transgenic lines and receptor in agronomic characters, such as plant height, 1 000-kernel weight and spike number per plant that were higher in transgenic lines than in receptor. Compared with in the receptor, in transgenic lines the plant height was from 79.2 cm to 83.8 cm and 13.4 cm higher; the average value of spike number per plant was 3.82 and higher significantly too; and 1 000-kernel weight was from 50.53 g to 52.98 g and 6.94 g higher. While the spike length, number of spikdet and grain number per spike were lower （Table 1）. （3） The mature date of CK was on June tenth, those of transgenic plants （03048,03039,03050） were on June 2, June 3, and June 6, respectively, which were 4 to 8 days shorter than that of receptor. It showed that anti-PLDγ gene was good to advance mature date. Complete male sterile was observed in T1 and the following generations and it would play an important role in heterosis utilization.

关 键 词：穗茎注射法 分子检测 整合 转基因小麦 

分 类 号：S512.1[农业科学—作物学]