hIFN-λ1(hIL-29)基因及在COS-7细胞中瞬时表达和抗病毒效应  

Instantaneous expression and antivirals effect of human IFN-λ_1 gene in COS-7 cells

作　　者：王建红[1] 马丽丽[1] 谢宇锋[1] 盛伟华[1] 缪竞诚[1] 吴康[1] 陈雄艳[1] 杨吉成[1] 

机构地区：[1]苏州大学医学院细胞与分子生物学教研室,苏州215007

出　　处：《中国免疫学杂志》2005年第10期744-746,751,共4页Chinese Journal of Immunology

基　　金：江苏省高校自然科学研究项目(01KJB180001)

摘　　要：目的:克隆hIFN-λ1基因,构建pcDNA3.1A-IFN-λ1表达质粒,并在真核细胞中获得表达,以进一步研究rhIFN-λ1的生物学特性。方法:采用RT-PCR法从病毒诱导的A549细胞mRNA中克隆hIFN-λ1基因,并重组于pcDNA3.1A真核表达载体上。结果:经双酶切和PCR鉴定及DNA序列分析,发现所克隆的基因与GenBank公布的序列一致,并在COS-7细胞中获得了有效瞬时表达。结论:成功克隆了hIFN-λ1基因,并成功地获得了瞬时表达,其rhIFN-λ1表达产物具有抗病毒活性。Objective： To clone and express hIFN-λ1 gene and construct the eukaryotic expressing vector pcDNA3.1A-IFN-λ1 , and study the biological activity of rhIFN-λ1 Methods： The cDNA fragment encoding hIFN-λ1 was amplified by RT-PCR from the total RNA extract- ed from virus-induced A549 cells. It was cloned into the eukaryofic expressing vector pcDNA3.1A. Results： With the identification of double enzyme map,PCR and sequencing,we found that gene we have coloned is in accordance with the gene sequence published in GenBank.The rhIFN-λ1 gene was expressed in COS-7 cells transiently. Condusion：The hIFN-λ1 gene was cloned successfully and expressed in COS-7 cells transiently, and its products have anti-virus activity.

关 键 词：λ1-干扰素 基因克隆 COS-7细胞 表达 

分 类 号：R977.6[医药卫生—药品] R978.7[医药卫生—药学]