日本血吸虫31/32kDa重组抗原细胞免疫机制  

Study on protective cell immune mechanism of recombinant 31/32kDa antigens of Schistosoma japanicum

作　　者：陈喜珪[1] 沈定文[1] 罗金萍[1] 覃金红[1] 彭胜国[1] 

机构地区：[1]湖北咸宁学院医学院寄生虫学教研室,咸宁437100

出　　处：《中国公共卫生》2005年第10期1197-1198,共2页Chinese Journal of Public Health

基　　金：湖北省教育厅2002年重点课题资助(2002A04004)

摘　　要：目的探讨细胞免疫在重组日本血吸虫31/32kDa抗原(rSj31/32)保护性免疫机制中的作用。方法采用rSj31/32抗原免疫BALB/c小鼠,ELISA分别检测免疫前后小鼠血清干扰素(IFN-γ)和白细胞介素(IL-4)的含量,攻击感染后以脾细胞培养法检测经刀豆蛋白(ConA)和可溶性虫卵抗原(SEA)刺激后,小鼠脾细胞分泌IFN-γ和IL-4的水平。结果rSj31/32抗原免疫后血清IFN-γ的水平明显增加(P<0.001),而IL-4的含量无明显变化;经SEA刺激,免疫组脾细胞产生较高水平的IFN-γ,而产生的IL-4水平较其他组低(P<0.01)。结论细胞免疫在rSj31/32诱导的保护性免疫中起着重要作用。Objective To study the effect of cell immunity in the protective immune mechanism of recombinant rSj31/ 32kDa antigens of Schistosoma japonicum（rSj31/32）. Methods BALB/c mice were immunized three times with rSj31/32. ELISA kits were used to examine the levels of IFN-γ and IL-4 before and 6 weeks after immunization. By the culture of spleen cells after challenge, IFN-γ and IL-4 after stimulation with SEA were quantified by ELISA kits. Results The level of IFN-γ were increased significantly after immunized with rSj31/32（P 〈 0.001）. There was no obvious change in the level of IL-4. With spleen cells from rSj31/32 vaccinated mice, IFN-γ was the most abundantly produced cytokine in response to SEA, while IL-4 secretion was the lowest（P〈0.01）. Conclusion Cell immunity might play an important role in the protective immunity elicited by rSj31/32 antigen.

关 键 词：日本血吸虫 rSj31/32 保护性免疫 细胞免疫 

分 类 号：R383.2[医药卫生—医学寄生虫学]