四氢巴马汀对豚鼠心室肌细胞跨膜内向Ca^(2+)电流的抑制作用(英文)  

Inhibitory effect of tetrahydropalmatine on calcium current in isolated cardiomyocyte of guinea pig

作　　者：徐春[1] 孙明智[1] 李玉荣[1] 杨宝峰[2] 王丽娟[3] 李金鸣[3] 

机构地区：[1]哈尔滨医科大学生理教研室,哈尔滨150086 [2]哈尔滨医科大学药理教研室,哈尔滨150086 [3]中国医科大学药理教研室

出　　处：《中国药理学报》1996年第4期329-331,共3页Acta Pharmacologica Sinica

摘　　要：目的:研究四氢巴马汀(THP)对豚鼠心室肌细胞膜Ca^(2+)通道的作用。方法:分离豚鼠心室肌细胞,利用膜片箝技术观察、记录心室肌细胞膜跨膜内向钙电流(I_(Ca))。结果:THP灌流5min后明显抑制豚鼠心室肌细胞内向I_(Ca),其作用呈现剂量-效应关系,并具有频率依赖性。0.1,1,10μmol·L^(-1)的THP使I_(ca)从1.15±0.22,0.91±0.18,1.60±0.42nA(control,n=5)分别降低到0.90±0.21(P<0.01),0.56±0.21(P<0.01),0.83±0.21(P<0.05)nA,抑制率分别为22％,38％和48％,THP的作用易于洗脱。峰值电压为0mV,此时THP作用最明显,THP对较高频率刺激产生的电流抑制作用较强,2Hz时的抑制率高于0.1Hz时的抑制率(P<0.01)。结论:THP具有Ca^(2+)通道阻滞作用。AIM: TO study the effect of tetrahydropalmatine (THP) on calcium channels in ventricular single cells of guinea pig heart. METHODS: Patch-clamp technique (whole cell recording) was used to observe calcium current in ventricular myocytes. RESULTS: THP decreased Ica in ventricular myocytes with a dose and frequency-dependent manner. THP (0.1, 1, and 10 μmol · L-1) decreased Ica from 1. 15 ± 0. 22, 0. 91 ±0.18, and 1. 60 ± 0. 42 nA (control) to 0. 9 ±0.21 (P<0.01), 0.56±0.21 (P<0.01), and 0.83±0.21 nA(P<0.05), respectively, number of cells is five in each group (n = 5), and the rates of the depression of Ica were 22 %, 38 %, and 48 %, respectively. The effect was easily reduced by washing the cell with the Tyrode's solution. The current-voltage relation curve showed that the potential producing peak value of Ica was 0 mV at which THP had the most markedly inhibited action on Ica When the stimulating frequency was changed, Ica varied in a frequency-dependent manner 5 min after THP was given, and the inhibition of THP was stronger at 2 Hz than that at 0.1 Hz. CONCLUSION: THP possessed a Ca2± channel blocking effect.

关 键 词：四氢巴马汀 心肌 钙通道 膜片箝技术 细胞培养 

分 类 号：R965.1[医药卫生—药理学] R972[医药卫生—药学]