高斯光束荧光共焦显微镜的三维光学传递函数  

The optical transfer function of a fluorescent confocal microscope with extended Gaussian source

作　　者：杨初平[1] 

机构地区：[1]华南农业大学理学院,广州510642

出　　处：《激光技术》2005年第5期552-554,共3页Laser Technology

基　　金：广东省自然科学基金资助项目(010297)

摘　　要：研究高斯光源的束斑半径、光源孔径和探测器孔径对荧光共焦显微镜3-D OTF的影响,获得了具有有限光源孔径、探测器孔径和束斑半径的荧光共焦显微镜的三维光学传递函数(3-D OTF)。数值计算结果表明,束斑半径影响到光源孔径和探测器孔径的选择,与采用平行光比较,采用小束斑半径的高斯光束可以提高光源孔径和探测器孔径,获得更好的分辨率和更高的信噪比。The influence of the three parameters,namely the beam size of Gaussian source, the source size and the detector size,on the three-dimensional optical transfer function （3-D OTF） of an incident light fluorescent confocal microscope is investigated, and the 3-D OTF of such a set-up is derived. The calculated results show that the beam size has an effect on choosing both the source size and the detector size by means of a gaussian beam with the smaller beam size, both the source size and the detector size can be increased, and better optical resolution and higher signal-noise-ratio can be reached, compared with those by means of a parallel beam.

关 键 词：信息光学 荧光共焦显微镜 高斯光束 束斑半径 

分 类 号：O438[机械工程—光学工程]