利用分子梳方法研究单个DNA-YOYO-1复合体的光漂白性质(英文)  

Investigating the Photobleaching Property of Single DNA-YOYO-1 Complex by Molecular Combing Method

作　　者：刘玉峰[1,2] 王鹏业[1] 窦硕星[1] 谢平[1] 刘玉颖[1] 刘全慧[3] 

机构地区：[1]中国科学院物理研究所软物质物理实验室 [2]湖南大学应用物理系,长沙410082 [3]湖南大学应用物理系

出　　处：《Chinese Journal of Chemical Physics》2005年第5期651-653,共3页化学物理学报（英文）

基　　金：ProjectsupportedbytheNationalNaturalScienceFoundationofChina(60025516,10334100).

摘　　要：利用分子梳方法结合荧光显微术对单个DNA-YOYO-1复合体的光漂白过程进行了研究,发现单个DNA-YOYO-1复合体的光漂白是一个随光照时间指数衰减的过程,并给出了DNA拓扑结构以及光照强度对光漂白过程的影响.The two dyes named YOYO and TOTO used as fluorescent probes in DNA experiments were reported in 1992. When the cyanine dye YOYO is bound to DNA its fluorescence quantum yield can be 1000-fold . higher than when it is free in solution . Because of this YOYO dye is widely used not only for detection of DNA in electrophoresis and visualization of DNA in fluorescence microscopy, but also for single molecular detection of DNA. On the other hand,

关 键 词：DNA YOYO 光漂白 分子梳 

分 类 号：Q71[生物学—分子生物学]