油松胚珠愈伤组织诱导和悬浮细胞系的建立  

Induction of Callus from the Ovule of Pinus tabulaeformis Carr and Establish- ment of Suspension Cell Line

作　　者：杨远媛[1] 贺窑青[1] 郑彩霞[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083

出　　处：《植物生理学通讯》2005年第5期591-594,共4页Plant Physiology Communications

基　　金：国家自然科学基金(30371144)

摘　　要：雌配子体处于游离核时期的油松胚珠以含不同浓度和配比生长调节剂的培养基诱导产生愈伤组织,并建立了悬浮细胞系。这一悬浮细胞系生长快,分散性好,稳定均一,适用于研究其生理、生化和细胞周期调控。Ovule callus of Pinus tabulaeformis Carr, which female gametophytes were on the mitosis of the free nuclei, was induced on MS media with different concentrations and combinations of hormones and the suspension cell line was established. The suspension cell line grew rapidly and was fairly homogenous. It was suitable to be studied on the mechanisms of the plant physiology, biochemistry and the regulation of cell cycle.

关 键 词：油松胚珠 愈伤组织 悬浮细胞系 愈伤组织诱导 胚珠 油松 生长调节剂 细胞周期调控 雌配子体 培养基 

分 类 号：S791.254[农业科学—林木遗传育种]