胶束液相色谱法测定血清中硝苯地平的含量  

Content Determination of Serum Nifedipine by Micell Liquid Chromatography

作　　者：陈丽娟[1] 张瑶纾[2] 

机构地区：[1]天津市胸科医院药剂科,天津市300051 [2]天津医科大学,天津市300070

出　　处：《中国药房》2005年第19期1482-1483,共2页China Pharmacy

摘　　要：目的:建立以胶束液相色谱法测定人血清中硝苯地平含量的方法。方法:色谱柱为C18,流动相为甲醇-水(60∶40,内含25mmol/L的SDS),检测波长为248nm,灵敏度为0.01AUFS,流速为1.0ml/min,记录纸速为2.1mm/min。结果:硝苯地平检测浓度在4～40ng/ml范围内线性关系良好(r=l.0002),平均回收率为98.15%(RSD=1.49%)。结论:本方法操作简便、准确、易行、重现性好,可为硝苯地平的临床血药浓度监测提供一种新的方法。OBJECTIVE： To establish micell liquid chromatography for the content determination of nifedipine in human serum. METHODS： The determination was performed on C18 chromatographic column, the mobile phase consisted of methanol- water（60 ： 40, which containing 20mmol/ L SDS）, the detection wavelength was 248nm, the sensitivity was 0.01AUFS, the flow rate was 1.0ml/min and the chart speed was 2. lmm/min. RESULTS：The linear range for nifedipine was 4-40ng/ml（r= 1.0 002）, the average recovery was 98.15% （RSD = 1.49%） .CONCLUSION： The method is simple, accurate, feasible, reproducible,and it can be used as a new method for the monitoring of clinic haemoconcentration of nifedipine.

关 键 词：胶束液相色谱法 硝苯地平 血清 含量 

分 类 号：R927.2[医药卫生—药学]