香果树组织培养技术研究  

Study on Tissue Culture Technique of Emmenopterys henryi

作　　者：韦小丽[1] 朱忠荣[1] 廖明 金天喜 

机构地区：[1]贵州大学林学院,贵阳550025 [2]贵州省林业厅,贵阳550001

出　　处：《种子》2005年第10期27-29,共3页Seed

基　　金：贵州省林业厅攻关课题(2001-09)

摘　　要：采用香果树幼树叶片作外植体,接种到附加不同浓度的KT和ZT组合的MS培养基上,诱导植株再生。结果表明,最适宜香果树叶片愈伤组织诱导和芽增殖的培养基是MS+KT 1.0+ZT 2.0,愈伤组织诱导率达35.6%,芽增殖倍数为2.8倍;比较理想的生根培养基是1/2 MS+IAA 0.5和1/2MS+IBA 0.5,生根率分别为65%和75%。试管苗移栽最理想的移栽基质是泥炭与珍珠岩(5∶1)的混合物,移栽成活率达95.2%。The leaves from young trees ofEmmenopterys henryi were inoculated onto MS supplemented with various concentrations combination of KT （kinetin） and ZT （Zeatin）, in order to induce regeneration. The resuits showed that MS ＋ KT 1.0 ＋ ZT 2.0 is suitable for inducing callus and bud multiplication, the inducing rate of callus is 35.6%, the bud multiplication reach 2.8 times. The 1/2 MS ＋ IAA 0.5 and 1/2 MS ＋ IBA 0.5 are suitable for inducing root formation, respectively with 65 % and 75 % rooting rate. The most suitable transplanting media of test-tube seedling is the mixed media of peat and perlite at the rate of five to one, the survival rate of transplanting seedlings is 95.2%.

关 键 词：香果树 组织培养 叶 培养基 组织培养技术 愈伤组织诱导率 MS培养基 果树叶片 生根培养基 试管苗移栽 

分 类 号：S68[农业科学—观赏园艺] S759.9[农业科学—园艺学]