萃取淫羊藿挥发油的实验与分析  

Analysis of essential oil from epimedium brevicornum maxim by GC-MS and its orthogonal experiment of supercritical fluid extraction

作　　者：回瑞华[1] 侯冬岩[1] 李铁纯[1] 刘晓媛[1] 

机构地区：[1]鞍山师范学院分析测试研究中心,鞍山114005

出　　处：《分析试验室》2005年第10期63-66,共4页Chinese Journal of Analysis Laboratory

基　　金：辽宁省教育厅科学技术基金资助课题(20331079)

摘　　要：报道了用正交实验法研究超临界萃取淫羊藿挥发性成分的条件;结果显示最佳萃取条件为萃取压力30 MPa,萃取温度40℃,萃取时间1 h。按对结果影响大小依次排列为:萃取压力→萃取温度→萃取时间,在最佳条件下萃取挥发油,收率为2.7%;并用气相色谱-质谱联用技术分析了淫羊藿挥发油的化学成分,从中确认出43种化学成分,用峰面积归一化法通过化学工作站数据处理系统得出各化学成分在挥发油中的质量分数;其中主要成分为薄荷醇、1,2-二甲氧基-4-(2-丙烯基)-苯、5-(1-丙烯基)-1,3-苯并间二氧杂环戊烯、3,5-二甲氧基-甲苯、冰片、十五(碳)烷、1,2,3-三甲氧基-5-甲苯、外-葑醇、2,6,6-三甲基-2,4-环庚二烯-1-酮、2-莰酮等。Orthogonal design of 3 factors at 3 levels was applied to optimization of supereritical CO2 fluid extraction conditions of essential oil from Epimedium brevicornum Maxim. The optimal conditions of extraction pressure 30 MPa, extraction temperature 40℃ and extraction time 1h were obtained. The sequence according to the effect of the extraction results is as follows ： extraction pressure→extraction temperature→extraction time. Constituents of the essential oil were analyzed by gas chromatography-mass spectrometry, and 43 compounds were identified, their relative contents were determined by peak area normalization method. The main constituents were found to be menthol, 1, 2-dimethoxy-4-（2-Propenyl）-benzene, 5-（1-Propenyl）-1, 3-benzodioxole, 3, 5- dimethoxy-toluene, bomeol, pentadecane, 1,2,3-Trimethoxy-5-methyl-benzene, exo-fenchol, 2,6,6-trimethyl-2,4-cycloheptadien-1-oen, comphor, etc.

关 键 词：淫羊藿 挥发油 萃取实验 气相色谱/质谱分析 

分 类 号：Q949.783.2[生物学—植物学] R248.1[医药卫生—中医临床基础]