基于介电泳的生物粒子分离芯片  

Research on bio-particle separation chip based on dielectrophoresis

作　　者：倪中华[1] 朱树存[1] 

机构地区：[1]东南大学机械工程系,南京210096

出　　处：《东南大学学报（自然科学版）》2005年第5期724-728,共5页Journal of Southeast University：Natural Science Edition

基　　金：国家自然科学基金资助项目(50475047);江苏省自然科学基金资助项目(BK20055072)

摘　　要：在阐述介电泳基础理论研究成果基础上,研究了传统介电泳力、电动旋转介电泳力和行波介电泳的计算模型力,分析了克劳修斯莫索提因子对正负介电泳的影响,给出了介电泳力的统一计算模型.研究了基于介电泳技术的多种类型的生物粒子分离芯片的研究现状,并从与芯片实验室的集成性、分离的准确性、分离对象多样性和分离对象的尺寸等综合指标,分析了基于微流体和介电泳混合作用、行波介电泳以及介电笼等方法构造的生物粒子分离芯片各自存在的问题,在此基础上提出综合应用行波介电泳和介电笼分离技术,建立面向200nm^2μm的微纳米生物粒子分离芯片,从而为建立满足分子芯片实验室需求的分离芯片研制提供了一个可行的方案.On the basis of introducing the research achievements of the basic theory of dieletrophoresis, the calculation model for dieletrophoresis-trapping （DEP-trapping）, rotatical-DEP （ROT- DEP）, traveling-wave-dielectrophoresis （TW-DEP） and general-DEP was discussed. And the effect of Clausius-Mossotti factor on positive, negative DEP was also analyzed. This paper deals with the current research status of many kinds of dieletrophoresis technology-based bio-particle separation chip, analyzes the respective problems of DEP-FFF, TW-DEP and DEP-Cage methods from the comprehensive indices of the integration of lab-on-chip, the correctness of separation, the object variety of separation, and the object size of separation etc. Finally, a separation technology of comprehensively applying DEP-cage and TW-DEP is proposed to construct the 200 nm to 2μm micro-nanobio-particle-oriented separation chip, and a feasible scenario is put forward for developing the separation chip meeting the requirement of lab-on-chip.

关 键 词：传统介电泳 行波介电泳 电动旋转介电泳 分离芯片 芯片实验室 

分 类 号：R318.11[医药卫生—生物医学工程]