心肌细胞外钙内流对PTEN表达的影响  

The effects of calcium influx induced by AngⅡ on PTEN expression

作　　者：周裔忠[1] 祝善俊[1] 于林君[1] 田颖[1] 王江[1] 

机构地区：[1]第三军医大学新桥医院心血管内科,重庆400037

出　　处：《中华老年心脑血管病杂志》2005年第4期257-259,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(30370584)

摘　　要：目的探讨血管紧张素Ⅱ(AngⅡ)介导的心肌细胞外钙内流对PTEN mRNA和蛋白表达的影响.方法新生Wistar乳鼠原代培养.用不同浓度的AngⅡ(10-8～10-5mol/L)促进心肌细胞外钙内流,特异的钙敏感指示剂Fur-2/AM测定心肌细胞内钙浓度([Ca2+]i).逆转录酶链式反应(RT-PCR)、Western blot分别检测PTEN mRNA和蛋白表达.结果AngⅡ在10-8～10-5mol/L浓度范围内能浓度依赖性促进心肌细胞外钙内流;心肌细胞外钙内流的增加能升高其PTEN mRNA和蛋白表达.结论心肌细胞内钙升高在促进心肌肥厚的同时,激活了PTEN的表达,可能对细胞内钙负荷所致的心肌肥厚起负反馈调节作用,PTEN是一内源性心肌肥厚抑制因子.Objective expression. Methods To investigate the effects of calcium influx induced by angiotensin （Ang Ⅱ ）on PTEN Culture of cardiomyocytes from Wistar rats aged 1 - 3 days was used. Intracellular calcium concentration（ [ Ca^2＋ ] i）was measured by Fur-2/AM, PTEN mRNA and protein expression by RT-PCR and western blot, respectively. Results Ang Ⅱ increased [ Ca^2＋ ] i of cardiomyocyte. PTEN mRNA and protein expression were increased with the increase in [ Ca^2＋ ] i of cardiomyocytes. Conclusions Intracellular calcium can trigger PTEN expression. PTEN may be a pivotal negative regulator of cardiomyocyte hypertrophy

关 键 词：血管紧张素Ⅱ 心肌病 肥厚性 钙信号 基因表达 

分 类 号：R541[医药卫生—心血管疾病]