新生儿缺氧缺血性脑病外周血T细胞亚群和膜白介素-2受体表达及临床意义  

The expression of T subsets and mIL-2R in the peripheral blood of newborns with HIE

作　　者：王健[1] 陆勤[2] 

机构地区：[1]安徽理工大学医学院病原学与免疫学教研室,淮南市232001 [2]安徽省淮南市妇幼保健院新生儿科,230001

出　　处：《中国实用儿科杂志》2005年第10期608-610,共3页Chinese Journal of Practical Pediatrics

基　　金：淮南市科技局自然科学重点项目(编号:2001-05)

摘　　要：目的观察新生儿缺氧缺血性脑病(HIE)患儿外周血T细胞亚群和膜白介素-2受体(mIL-2R)表达,探讨其临床意义。方法安徽省淮南市妇幼保健院新生儿科2002年5月至2003年10月收治HIE患儿32例。以Ficoll-Hypaque常规分离外周血单个核细胞(PBMC),用生物素-链霉亲和素(BSA)系统检测新生儿HIE及足月正常新生儿生后第1、3、7日CD3+、CD4+、CD8+、CD4+/CD8+阳性率,及PHA诱导前后mIL-2R表达水平。结果新生儿HIE患儿生后第1日CD3+、CD4+、CD8+阳性率及CD4+/CD8+、静息期和诱导期mIL-2R表达水平与正常对照组相比,差异有显著性(P<0.01或P<0.05);生后第3日与正常对照组相比,差异有显著性(P<0.05);生后第7日CD3+、CD4+、CD8+与正常对照组相比,差异有显著性(P<0.05),CD4+/CD8+与正常对照组相比,差异无显著性(P>0.05)。在不同程度HIE患儿中,以重度HIE患儿细胞免疫功能异常最明显。结论新生儿免疫细胞幼稚、未分化成熟、表达水平偏低,HIE病理过程有细胞免疫的改变及参与,HIE患儿存在一定程度的细胞免疫功能紊乱。Objective To observe the expression level of T subsets and membrane interleukin-2 receptor （mIL-2R） in the peripheral blood of newborns with hypoxic ischemic encephalopathy （ HIE） and its clinical manifestation. Methods The peripheral blood mononuclear cells （ PBMC ） of newborns with HIE and normal controls were isolated by routine Ficoll-Hypaque, and its level of CD3^＋ , CD4^＋ , CD8^＋ , CD4^＋/CD8^＋ and mIL-2R induced and not induced by PHA were detected by biotin-streptavidin （ BSA） on spot of the first,third and seventh day. Results After born on spot of the first day,the positive rates of CD3^＋ , CD4^＋ , CD8^＋ CD4^＋/CD8^＋ and mIL-2R induced and not induced were significantly higher in HIE groups than those in normal controls （ P 〈 0. 01 - P 〈 0. 05）. After newborns being born three days,there were significant differences in HIE groups compared with those in normal controls （ P 〈 0. 05）. After newborns being born seven days,the positive rates of CD3^＋ , CD4^＋ , CDs were significantly different in HIE groups from those in normal controls （ P 〈 0. 05）. The levels of CD4^＋/CD8^＋ and mIL-2R induced and not induced by PHA were not significantly different in HIE groups and normal controls （ P 〉 0. 05 ）. The most infantile cellular immune function was existent in newborns with HIE （ severe ）. Conclusion The main character of peripheral blood mononuclear cells of newborns is all infantile and undifferentiated,whose surface sign is infantile,and a few cellular immune can take part in course of HIE. Certain cellular immune function disorder can be in newborns with HIE.

关 键 词：新生儿 缺氧缺血性脑病 T细胞亚群 膜白介素-2受体 生物素-链霉亲和素 

分 类 号：R72[医药卫生—儿科]