姜黄素对前列腺特异抗原基因表达的抑制  

Inhibition of the expression of prostate specific antigen by curcumin

作　　者：杨磊[1] 张莲英[1] 陈蔚文[1] 孔峰[1] 张鹏举[1] 胡晓燕[1] 张建业[1] 崔福爱[1] 

机构地区：[1]山东大学医学院生物化学与分子生物学研究所,山东济南250012

出　　处：《药学学报》2005年第9期800-803,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30171026).

摘　　要：目的研究姜黄素对前列腺特异抗原(PSA)表达的影响。方法化学发光法检测不同浓度的姜黄素处理前列腺癌细胞株LNCap后PSA含量,利用荧光素酶报告基因pGL3构建含PSA基因5′侧启动子区640bpDNA的荧光素酶表达载体pGL3PSA,并将其转染LNCap细胞,不同浓度姜黄素作用24h后应用荧光素酶测定系统检测荧光素酶表达活性。Westernblotting检测姜黄素处理过的LNCap细胞雄激素受体(AR)的表达。结果姜黄素抑制PSA、荧光素酶和AR受体的表达,并有浓度依赖性。结论姜黄素通过抑制雄激素受体的表达减弱对PSA基因启动子的作用,从而抑制PSA的表达。Aim To study the effect of curcumin on the expression of prostate specific antigen （PSA）. Methods AXSYM system-chemical luciferase method was used to examine the content of PSA in prostate cancer cell lines, LNCap after treated with different doses of curcumin, pGI2-PSA luciferase expression vector, containing 640 bp DNA of PSA gene 5＇ promoter region was constructed and transfected into LNCap cell with lipofectin. Through detecting the activity of luciferase, the effect of curcumin on the promoter of PSA was studied. Western blotting was used to detect expression of androgen receptor （AR） in LNCap cell with different concentrations of curcumin. Results The expression of PSA was inhibited and activity of luciferase was reduced by curcumin. There was also significant difference in AR expression as shown by Western blotting experiment after treatment of different doses of curcumin. Conclusion Through inhibiting AR expression, curcumin reduced the function of PSA promoter and inhibited PSA protein expression.

关 键 词：姜黄素 雄激素受体 前列腺特异抗原 前列腺癌 

分 类 号：R282.71[医药卫生—中药学] R737.25[医药卫生—中医学]