岩黄连注射液的高效液相色谱质谱联用指纹图谱研究  

HPLC fingerprint for the Corydalis saxicola Bunting injection

作　　者：刘思明[1,2] 胡坪[1] 罗国安[1,2] 王义明[3] 陈令新[3] 

机构地区：[1]华东理工大学化学与制药学院中药现代化工程中心 [2]清华大学药物研究所,北京100084 [3]清华大学药物研究所

出　　处：《药学学报》2005年第9期846-849,共4页Acta Pharmaceutica Sinica

基　　金：科技部重大技术标准专项资助项目(2002BA906A293).

摘　　要：目的建立岩黄连注射液的指纹图谱。方法色谱部分采用高效液相色谱梯度洗脱法,色谱柱为Kromasil10010C18柱(250mm×4.6mmID,5μm);流动相为乙腈和水;流速为0.5mL·min-1;在254nm波长下进行分析。质谱部分采用电喷雾离子阱质谱分析,正离子扫描方式。结果通过LC/MS对其中的11种主要成分进行了指认,LC/UV对其中的2种成分进行了定量评价。结论所建指纹图谱能体现岩黄连注射液的特点,相似度评价和方法学考察结果良好,可用于质量控制及定量测定。Aim To establish the fingerprint of Corydalis saxicola Bunting injection. Methods It was performed by HPLC Kromasil column was used with acetonitrile and H20 as mobile phase. The flow rate was 0. 5 mL·min^-1 The detection wavelength was 254 nm. The mass spectrometry detection was performed on a trap equipped with an ESI interface and operated in positive-ionization mode. Results Eleven components of Corydalis saxicola Bunting were identified by LC-MS and 7 of them were quantified. Conclusion The fingerprint chromatogram could represent the characteristics of Corydalis saxicola Bunting injection. Evaluation of resemblances and results of precision were satisfactory. This method could be used as quality and quantity control.

关 键 词：HPLC/UV/MS 指纹图谱 岩黄连注射液 

分 类 号：R917[医药卫生—药物分析学]