湖北贝母的HPLC指纹图谱分析  

HPLC fingerprint of Fritillaria hupehensis

作　　者：张国欣[1] 李娟[1] 张鹏[1] 阮汉利[1] 张勇慧[1] 皮慧芳[1] 吴继洲[1] 

机构地区：[1]华中科技大学同济医学院药学院,湖北武汉430030

出　　处：《药学学报》2005年第9期850-853,共4页Acta Pharmaceutica Sinica

基　　金：国家中医药管理局新药开发专项课题资助项目(D2X004A);湖北大学中药生物技术省重点实验室开放基金资助项目.

摘　　要：目的建立湖北贝母的指纹图谱分析方法。方法采用高效液相色谱蒸发光散射检测法以HypersilODS(250mm×4.6mmID,5μm)为色谱柱;流动相:甲醇(含0.05%三乙胺)水梯度洗脱;流速:1.0mL·min-1;记录时间:60min;以蒸发光散射检测器(ELSD)检测,检测条件:漂移管温度75℃,氮气流速1.9L·min-1。结果用梯度洗脱得到的色谱图各色谱峰分离较好,达到指纹图谱要求。结论为更好的控制湖北贝母的内在质量提供了可靠的分析方法。Aim To establish a fingerprint analysis method of Fritillaria hupehensis. Methods The fingerprint was performed by HPLC-ELSD. Hypersil ODS column was used; the mobile phase was composed of methanol （with 0.05% triethylamine） and water with gradient elution; flow rate was 1.0 mL · min^-1; recording time was 60 min; drift tube temperature was 75℃; gas flow rate was 1.9 L · min^-1. Results HPLC fingerprint of FritiUaria hupehensis was obtained. Conclusion A reliable method was provided for controlling the quality of Fritillaria hupehensis.

关 键 词：湖北贝母 指纹图谱 高效液相色谱-蒸发光散射法 

分 类 号：R917[医药卫生—药物分析学]