白花败酱草中异牡荆苷和异荭草苷的高速逆流色谱分离和制备  

Isalation and Separation Isoorientin and Isovitexin from Patrinia Villosa Juss by High Speed Counter-current Chromatography

作　　者：彭金咏[1] 范国荣[1] 吴玉田[1] 陈洪渊[2] 

机构地区：[1]第二军医大学药学院上海市药物(中药)代谢产物研究重点实验室,上海200433 [2]南京大学分析科学研究所,南京210093

出　　处：《分析化学》2005年第10期1389-1392,共4页Chinese Journal of Analytical Chemistry

基　　金：国家高新技术研究发展计划863计划基金资助项目(No.2003AA2Z3524)

摘　　要：应用高速逆流色谱分离制备白花败酱草中的异荭草苷和异牡荆苷,以乙酸乙酯∶乙醇∶水(4∶1∶5)为两相溶剂系统,上相为固定相,下相为流动相,流速2.0 mL/m in,主机800 r/m in,检测波长254 nm。以此分离条件经一步洗脱,从300 mg白花败酱草粗提物中制备得到异荭草苷24.1 mg和异牡荆苷49.8 mg,产物纯度经HPLC检测高达98.0%,结构经UV、IR、MS1、H NMR和13C NMR鉴定,二者均为首次从败酱属植物中分离得到。A semi-preparative high speed counter-current chromatography with a two-phase solvent system composed of ethyl acetate-ethanol-water （4：1：5, V/V） was used to isolate and separate isoorientin and isovitexin from Patrinia Villosa Juss. The mobile phase was the lower phase, and operated at a flow rate of 2.0 mL/min, while the apparatus rotated at 800 r/min and the effluent was detected at 254 nm. The separation produced 49.8 mg isovitexin and 24.1 mg isoorientin with purities of over 98.0% determined by high performance liquid chromatogrpahy in one step elution from 300 mg crude extract, and identification was performed by ultraviolet, infrared, mass spectrometry, ^1H nuclear magnetic resonance （NMR） and ^13C NMR. It is the first report of discovering isoorientin and isovitexin from the plant of Patrinia genius.

关 键 词：制备色谱 高速逆流色谱 异牡荆苷 异荭草苷 白花败酱草 分离制备 色谱分离 败酱草 牡荆 白花 

分 类 号：TQ461[化学工程—制药化工]