己烯雌酚印迹分子聚合物合成及其在残留分析中的应用  

Synthesis of Diethylstilbestrol-based Molecularly Imprinted Polymers and Its Application in Residues Analysis

作　　者：马金余[1] 陈波[1] 姚守拙[1] 刘程 

机构地区：[1]湖南师范大学化学生物学及中药分析省部共建教育部重点实验室,长沙410081 [2]湖南纽尔科技有限公司,长沙410007

出　　处：《分析化学》2005年第10期1413-1416,共4页Chinese Journal of Analytical Chemistry

基　　金：国家"十五"重大科技专项"食品安全关键技术"基金资助项目(No.2001BA804A39)

摘　　要：以己烯雌酚(D iethylstilbestrol,DES)为模板分子,α-甲基丙烯酸为功能单体,二甲基丙烯酸乙二醇酯为交联剂,合成己烯雌酚分子印迹聚合物;以该聚合物为填料制成固相萃取小柱,应用于己烯雌酚残留分析的样品前处理,并比较了该固相萃取小柱与C18固相萃取小柱对DES保留行为的差异。通过选择不同浓度的甲醇水溶液,己烯雌酚达到理想的分离纯化效果;对加标鸡肉样进行了含量测定,回收率可达95%以上。Using diethylstilbestrol （DES） as template molecule, α-methacrylic acid （MAA） as functional monomer, and ethylene dimethacrylate （EDMA） as crosslinking agent, a polymer was synthesized by moleeular imprinting technique. The molecularly imprinted polymers （MIPs） were loaded into the solid-phase extraction （SPE） cartridge, which was applied to the pretreatment of residues analysis of diethylstilbestrol. Elution conditions of DES on the solid-phase extraction cartridge were optimized. Furthermore, the difference of the retention characteristics of diethylstilbestrol on the C18 and DES-MIPs solid-phase extraction cartridge was compared. The DES-MIPs-SPE had higher selectivity than the C18-SPE. The method has been applied to pretreat spiked samples successfully with the recovery more than 95%.

关 键 词：分子印迹聚合物 固相萃取 己烯雌酚 聚合物合成 残留分析 印迹分子 应用 二甲基丙烯酸乙二醇酯 Α-甲基丙烯酸 样品前处理 

分 类 号：O657.7[理学—分析化学] O631[理学—化学]