甲基莲心碱对豚鼠离体胆道平滑肌的作用  

Effect of Neferine on Isolated Biliary Smooth Muscles

作　　者：丁庆[1] 谭俊峰 张丽娟 郝战 夏家红 邹声泉[1] 胡文淑[1] 

机构地区：[1]武汉同济医科大学医疗系九○级一大班,武汉同济医科大学附属同济医院外科,武汉同济医科大学基础医学院药理学教研室

出　　处：《同济医科大学学报》1996年第4期364-366,共3页Acta Universitatis Medicinae Tongji

基　　金：湖北省自然科学基金

摘　　要：在离体正常和炎性胆囊，甲基莲心碱（Ｎｅｆ）明显抑制Ｋ＋所致收缩，其ＩＣ（５０）分别为１２０μｍｏｌ／Ｌ和５μｍｏｌ／Ｌ，表明Ｎｅｆ对炎性胆囊抑制作用较强。Ｎｅｆ和维拉帕米（Ｖｅｒ）可抑制Ｋ￣＋所致Ｏｄｄｉ括约肌两相收缩，Ｖｅｒ主要抑制持久相收缩，而Ｎｅｆ以抑制时相收缩为强。Ｎｅｆ和Ｖｅｒ非竞争性拮抗组织胺（Ｈｉｓｔ）所致胆囊收缩，其ＰＤ′＿２值分别为５．０１和５．３８。提示Ｎｅｆ对胆道平滑肌的抑制作用与其抗内钙释放有关。The contractions induced by K+ on isolated gallbladder of guinea pigs were significantly inhibited by neferine, IC(50)of neferine on normal and inflamed gallbladder were 120 μmol/L and 5μmol/L respectively, indicating that the inhibitory effect of neferine on normal gallbladder was weaker than that on inflamed ones, The initial phasic and the late tonic contractions induced by K+ on isolated Oddi's sphincter of guinea pigs were inhibited by neferine or verapamil.Verapamil showed mainly an inhibition of late phase, whereas neferine showed mainly an inhibition of the initial phase.Neferine and Verapamil can antagonize the contractions induced by histamine on isolated guinea pig gallbladder in a noncompetitive manner.The PD2 valubs of neferine and Verapamil were 5.01 and 5.38 respectively. The results suggest that the inhibitory effects of neferine on biliary smooth muscles may be due to its Ca(2+)-antagonism and the mode of its action being different from that of Verapamil.

关 键 词：莲心碱 甲基 平滑肌 胆道 莲子 

分 类 号：R285.5[医药卫生—中药学] R965.1[医药卫生—中医学]