甲基黄酮醇胺对分离的胎鼠脑细胞内游离钙浓度的影响(英文)  

Effects of methylflavonolamine on free intracellular calcium in isolated embryonic rat brain cells

作　　者：汤允昭[1] 段文贞[2] 

机构地区：[1]山西医学院药理教研室 [2]中国医学科学院药物研究所药理室

出　　处：《中国药理学报》1996年第4期306-308,共3页Acta Pharmacologica Sinica

摘　　要：目的:观察甲基黄酮醇胺(MFA)对胎鼠脑细胞内游离钙浓度在静息以及激动剂存在时的作用。方法:用钙离子荧光染料Fura 2-AM负载后,测定分离的胎鼠脑细胞内游离钙浓度([Ca^(2+)]_i)及其变化。结果:在含钙1.3mmoL·L^(-1)的Hanks’液中,[Ca^(2+)]_i为197±20nmol·L^(-1)(n=44)。MFA0.15mmol·L^(-1)对静息脑细胞内钙浓度无明显影响。在细胞外钙1.3mmol·L^(-1)条件下,MFA(0.03—0.3 mmoL·L^(-1))浓度依赖性地抑制高钾去极化导致的[Ca^(2+)]_i升高,IC_(50)为0.14(95％可信限:0.05—0.42)mmoL·L^(-1)。在较高浓度时,MFA(0.15—0.3mmoL·L^(-1))也可抑制谷氨酸兴奋所引起的[Ca^(2+)]_i,IC_(50)为0.20(95％可信限:0.01—3.40)mmoL·L^(-1)。结论:MFA抑制高钾去极化引起的[Ca^(2+)]_i升高,在较高浓度时也拮抗谷氨酸兴奋所致的[Ca^(2+)]_i升高。AIM: To observe the effects of methylflavonola-mine ( MFA ) on free intracellular calcium concentration ([Ca2+]|) of isolated embryonic rat brain cells in presence and absence of high extracellular potassium and L-glutamate. METHODS: [Ca2+], was measured in a spectro-fluorophotometer by preloading the cells with calcium sensitive fluorescent indicator Fura 2-AM. RESULTS: Resting [Ca2+ ], was 197 ± 20 nmol ·L-1 (n = 44) in the presence of Ca2+ 1.3 mmol ·L -1 in Hanks' solution. MFA 0.15 mmol·L -1 had no effect on the resting [ Ca2+]. When extracellular Ga2+ was 1.3 mmol·L-1, MFA (0.03 - 0.3 mmol · L-1 ) concentration-dependently inhibited the [Ca2+], elevation induced by high extracellular potassium, with an IC50 value of 0.14 (95 % confidence limits: 0.05-0.42) mmol·L-1. At higher concentration (0.15 - 0.30 mmol ·L-1), MFA decreased L-glutamate-induced [ Ca2+ ], elevation, with an IC50 of 0.20 (95 % confidence limits: 0.01 - 3.40) mmol· L-1. CONCLUSION: MFA inhibited Ca2+ influx through voltage-dependent calcium channel and, at higher concentration, through receptor-operated calcium channel in the embryonic rat brain celts.

关 键 词：甲基黄酮醇胺 FURA-2 钙 谷氨酸 脑 细胞 

分 类 号：R964[医药卫生—药理学] R965[医药卫生—药学]