足阳明经穴针刺对家兔离体胃窦平滑肌细胞舒缩活动与胞内三磷酸肌醇含量的影响  

Effects on the cell length and intracellular inositol triphos-phate content of in vitro smooth muscle cells in rabbit gastric antrum by acupuncture at the acupoints of the foot-Yangming channel

作　　者：邓元江 易受乡[2] 严洁[2] 林亚平[2] 郭晖[2] 向志勇[2] 刘卫英[2] 吴芳[2] 

机构地区：[1]广州中医药大学第二附属医院神经一科,广东省广州市510405 [2]湖南中医学院针灸推拿系,湖南省长沙市410007

出　　处：《中国临床康复》2005年第31期154-157,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助(30171136)~~

摘　　要：目的:探讨针刺血清足三里、天枢、梁门等足阳明经穴针刺血清对家兔离体胃窦平滑肌细胞舒缩活动及胞内三磷酸肌醇含量的影响。方法:实验于2003-07/12在广州中医药大学和广州中医药大学附属第二医院完成。将45只家兔随机分为5组,每组9只。生理盐水组:耳缘静脉滴注0.9%氯化钠溶液剂量为10mL/kg;阿托品模型组:耳缘静脉滴注以生理盐水配制的2.5mg/100mL的阿托品;足阳明经穴组、足少阳经穴组、足太阳经穴组均先静滴阿托品(滴注方法同阿托品模型组),在滴完阿托品总量的一半时,开始分别电针双侧足阳明经穴(足三里、天枢、梁门、四白)、足少阳经穴(阳陵泉、带脉、日月、阳白)、足太阳经穴(承筋、背下点、背上点、攒竹)30min,待电针完毕后,颈动脉采血6mL离心15min,取上清,制备成“针刺血清”。另取家兔10只,手术取胃,分离胃窦平滑肌,经Ⅱ型胶原酶消化后制备胃窦平滑肌细胞悬液,以“针刺血清”与家兔胃窦平滑肌细胞作用,用戊二醛固定后测定细胞长度和细胞内三磷酸肌醇含量。结果:进入细胞长度分析的家兔为43只,进入三磷酸肌醇含量分析家兔为45只。①细胞长度比较:足阳明经穴组明显低于足少阳经穴组犤(42.05±7.85,77.33±9.80)μm,(P<0.01)犦;足太阳经穴组明显高于生理盐水组犤(75.88±9.14,64.58±6.59)μm,(P<0.05)犦。②三磷酸肌醇含量比较:足少阳经穴组明显低于组阳明经穴组犤(33.15±7.45,48.96±8.89)pmol/106,(P<0.01)犦;阿托品模型组明显低于生理盐水组犤(31.65±7.69,40.84±9.82)pmol/106,(P<0.05)犦。结论:足阳明经穴针刺血清能使离体胃窦平滑肌细胞产生明显的收缩效应,其收缩效应的产生与升高细胞内的三磷酸肌醇含量有关。AIM： To investigate the effects of acupuncture serum at the acupoints of the foot-Yangming channel of foot-Sanli, Tianshu and Liangmen on the cell length and content of intracellular inositol triphosphate of in vitro smooth muscle cells in rabbit gastric antrum. METHODS： The experiment was carried out in Guangzhou University of Traditional Chinese Medicine and its Second Affiliated Hospital between July and December 2003. Forty-five rabbits were randomly divided into 5 groups with 9 rabbits in each group： saline group （the rabbits were treated with venous drip of 0.9% NaCl at ear edge with the dosage of 10 mL/kg）, atropine model group （the rabbits were treated with venous drip of 2.5 mg/ 100 mL atropine dispensed with saline at ear edge）, the foot-Yangming channel acupoints group, foot-Shaoyang channel acupoints group and footTaiyang channel acupoints group [the method of drip was the same as that in the atropine model group, and then the rabbits were given electroacupuncture at foot-Yangming channel acupoints （foot-Sanli, Tianshu, Liangmen and Sibai）, foot-Shaoyang channel acupoints （Yanglingquan, Daimai, Riyue and Yangbai） and foot-Taiyang channel acupoints （Chengjin, Beishangdian, Beixiadian and Cuanzhu） for 30 minutes respectively]. After electroacupuncture, 6 mL blood sample was collected from carotid artery and centrifugated for 15 minutes, and then supernatant was ohtained to prepare acupuncture serum. Another 10 rabbits were selected, the stomach was taken out to isolate the smooth muscle cells of gastric antrum, and the smooth muscle cell suspension of gastric antrum was prepared by digestion of type H collagenase. The acupuncture serum was mixed with the smooth muscle cells of rabbit gastric antrum, and then the cell length and content of intracellular inositol triphosphate were detected after fixation with glutaral. RESULTS： Finally, 43 rabbits were involved in the analysis of cell length, and 45 rabbits were involved in the analysis of intracellular inositol triphosphate co

关 键 词：针刺疗法 平滑肌 肌细胞 阳明经 

分 类 号：R54[医药卫生—心血管疾病]