诺氟沙星侧脑室点燃大鼠癫痫及脑超微结构变化  

Kindling of rat epilepsy by intracerebroventricular injection of norfloxacin and ultrastructural changes of brain

作　　者：钱元恕[1] 陆杰[2] 黄仲荪[2] 范维正[2] 徐晨[3] 陈永[4] 

机构地区：[1]重庆医科大学第一医院传染科 [2]重庆医科大学第一医院基础部生理教研室,重庆630042 [3]重庆医科大学第一医院电镜室,重庆630042 [4]西南师范大学生物系

出　　处：《中国药理学报》1996年第4期373-375,共3页Acta Pharmacologica Sinica

摘　　要：目的:观察诺氟沙星对EEG的影响和对脑组织超微结构的毒性作用。方法:大鼠右侧脑室注射诺氟沙星150和300μg·kg^(-1)后,于皮层感觉运动区记录EEG并观察脑组织超微结构的变化。结果:大鼠EEG均出现痫样放电,两组大鼠右侧和左侧棘波发生时间为168±129,51±35s和276±138,118±65s。以注射侧较早发生,波形多变,幅度和频率逐渐增大,并伴肢体抽搐的行为学改变。电镜下,300μg·kg^(-1)组大鼠双侧大脑皮层、海马及小脑均有明显的神经元变性,包括高尔基复合体、粗面内质网及神经毡的髓样变性、细胞质肿胀、细胞核及染色质溶解、胶质细胞坏死等,并出现亮神经元和暗神经元改变,以暗神经元为著。结论:诺氟沙星点燃癫痫后,大鼠脑组织超微结构受到广泛损伤。AIM: To study the toxic effects of norfloxacin (Nor) on EEG and ultrastructure of the brain. METHODS: EEG was recorded in the sensorimotor area of rat cortex after Nor 150 and 300 μg·kg-1 were injected into the right ventricle of the brain. RESULTS: Spike waves occurred, first at the ipsilateral side and then bilaterally. The seizure spike appeared at 168± 129, 51 ±35 s, and 276± 138, 118 ± 65 s in the right and left cerebral cortices in 150 and 300 μg · kg-1 groups, respectively. The wave forms were variable, the wave amplitude and frequency increased gradually, accompanied with limb twitching. Neuron degeneration was seen in both cerebral hemispheres, hippocampus, and cerebellum 120 min after the icv of Nor 300 μg · kg-1: myeloid degeneration of Golgi complex, rough endoplasmic reticulum, and neuropils; swelling of cytoplasm; dissolution of nuclears and chromatin; necrosis of gliocytes and the appearance of changes in both the light and dark neurons, especially the dark ones. CONCLUSION: Rat brain was widely damaged after the kindling by Nor.

关 键 词：诺氟沙星 癫痫 脑室 脑电描记术 电子显微镜检查 

分 类 号：R965.1[医药卫生—药理学] R964[医药卫生—药学]