糖尿病大鼠视网膜微血管变化与血液流变学的关系  

Relationship between the changes of retinal microvessel and hemorrheology in diabetic rats

作　　者：何剑峰[1] 杜娟[2] 唐柳萍[2] 李向荣[2] 黄艳霞[2] 

机构地区：[1]广西医科大学第一附属医院眼科,广西壮族自治区南宁市530021 [2]广西医科大学第五附属医院眼科(柳州市人民医院眼科),广西壮族自治区柳州市545001

出　　处：《中国临床康复》2005年第31期93-95,共3页Chinese Journal of Clinical Rehabilitation

基　　金：广西自然科学基金资助(0007036)~~

摘　　要：目的:观察糖尿病大鼠早期视网膜毛细血管的变化情况及其与血液流变学的关系。方法:实验于2003-03/2004-03在中山大学医学动物实验中心完成。选取无菌级成年纯系雄性Wistar大鼠30只,随机分为糖尿病组和对照组,15只/组。糖尿病组腹腔注射链脲佐菌素65mg/kg(用0.1mol/L、pH4.5柠檬酸盐缓冲液配制)诱发糖尿病,对照组腹腔注射等量柠檬酸盐缓冲液。于糖尿病发病后4,6,12周,将两组大鼠空腹麻醉后尾静脉采血进行糖化血红蛋白、全血黏度、血浆黏度、红细胞压积、纤维蛋白原、红细胞聚集性及血液流变触性等检测,制备视网膜血管铺片,常规过碘酸-Schiff染色法染色后,光学显微镜观察,计数10个高倍镜视野的视网膜微血管壁的细胞数。空腹血糖检测采用葡萄糖氧化法,糖化血红蛋白检测用微柱层析法,全血黏度、血浆黏度、红细胞压积、纤维蛋白原的测定用SDZ-3型自动电子计时黏度计,红细胞聚集性和血液流变触性的测定采用LG-B-190型红细胞聚集仪。结果:实验共纳入30只大鼠,全部进入结果分析。①两组大鼠腹腔注药前后血糖变化:腹腔注药前,两组大鼠空腹血糖均低于6.5mmol/L(t=1.6149,P>0.05),尿糖定性均为阴性。腹腔注药后第1,3,7天,糖尿病组大鼠血糖均持续高于19mmol/L,且尿糖持续阳性,而对照组大鼠血糖均低于6.5mmol/L以下,尿糖阴性。注药后两组大鼠血糖具有显著性差异(t=5.6482～5.6694,P<0.01)。②糖尿病发病后不同时间两组大鼠视网膜血管形态观察结果:糖尿病组发病后4周,毛细血管行走尚规则,血管壁细胞数目及血管形态无明显异常改变;发病后6周,血管壁细胞数目减少;发病后12周,血管壁细胞数进一步减少,毛细血管迂曲,管径粗细不一,毛细血管呈囊样扩张。对照组在以上各实验时点毛细血管行走规则,管径粗细均一,视网膜毛细血管无异常改变。③糖尿病发病后不同时�AIM： To observe the relationship of the changes of retina blood capillary in the earlier period with hemorrheology in diabetic rats. METHODS： The experiment was carried out in the animal experimental center of Sun Yat-sen University from March 2003 to March 2004. Thirty germ-free adult pure male Wistar rats were selected, and divided randomly into two groups： diabetes group and control group with 15 in each group. The rats in the diabetes group were injected with 65 mg/kg streptozotocin through abdominal cavity （dispensed by 0.1 mol/L, pH 4.5 citrate buffer） to induce diabetes, and those in the control group were injected with ,the same dosage of citrate buffer through abdominal cavity. At the 4, 6 and 12 weeks after the onset of diabetes, the blood was gained from caudal vein after anesthesia in rats with an empty stomach in the two groups to perform the detection of glycosylation hemoglobin （HbAlc）, blood viscosity, plasma viscosity, hematocrit, fibrinogen, erythrocyte aggregation and blood thixotropy etc. The stretched preparation of blood vessel of retina was made, and after routine iodic acid-Schiff staining, observed by the light microscope, under 10 high magnification fields the number of cells of microvessel of retina was counted. The fasting blood glucose （FBG） was detected by glucose oxidase detection; HbAle was detected by microcylinder chromatography; . The blood viscosity, plasma viscosity, hematocrit and fibrinogen were measured by SDZ-3 auto-electron viscosimeter. The erythrocyte aggregation and blood thixotropy were measured by LD-B-190 aggregatimeter. RESULTS： Thirty rats were involved in the experiment and were all involved in the result analysis. ① Changes of blood sugar in rats in the two groups before and after abdominal cavity injection： Before injection, the blood sugar in the rats with an empty stomach in the two groups was lower than 6.5 mmol/L（t=.614 9,P 〉 0.05）, and the urine sugar was negative. At the 1^th, 3^rd and 7^th days after abdominal cavity injecti

关 键 词：血液流变学 毛细血管 视网膜 糖尿病血管病 大鼠 

分 类 号：R587.1[医药卫生—内分泌]