神经生长因子在培养的皮质神经细胞中抑制谷氨酸毒性  

Nerve growth factors prevent glutamate toxicity in cortical neuronal cultures 1

作　　者：林凌[1] 张鹤云[2] 顾海明[1] 汤国枝[2] 张祖暄[1] 王金晞 张维宁[4] 陈荣三 

机构地区：[1]南京大学医学院 [2]南京大学医学院生物化学系 [3]国家配位化学重点实验室 [4]南京大学医学院生物系

出　　处：《中国药理学报》1996年第3期221-224,共4页Acta Pharmacologica Sinica

摘　　要：目的：确定ＮＧＦ是否防止原代培养的神经细胞中谷氨酸引起的损伤．方法：采用皮质神经细胞体外培养及形态学观察，测定神经细胞的生存力和ＬＤＨ的释放来分析ＮＧＦ的作用，并利用钙指示剂Ｆｕｒａ２来检测［Ｃａ２＋］ｉ的变化．结果：ＮＧＦ阻止［Ｃａ２＋］ｉ的增加，并且对谷氨酸引起的皮质细胞的损伤具有拮抗作用，最大拮抗剂量为６０μｇ·Ｌ－１．结论：ＮＧＦ通过稳定［Ｃａ２＋］ｉ水平，或阻止［Ｃａ２＋］ｉ的升高来保护大脑皮质细胞抗谷氨酸毒性．IM: To determine if nerve growth factors (NGF) can protect against glutamate induced cortical neuron damage. METHODS: Neuron viability and lactate dehydrogenase (LDH) efflux in the bathing medium in primary cultures from 17 d old mouse fetal cortex were measured to assay NGF effect. Imaging of the calcium indicator dye Fura 2 was used to measure the [Ca 2+ ] i. RESULTS: The LD 50 for NGF free glutamate was 0 2 mmol·L -1 (95 % confidence limits 0-1 6 mmol·L -1 ). In the presence of NGF 60 μg·L -1 , 59 % of the neurons survived in glutamate 1 6 mmol·L -1 . The protective effect afforded by NGF was maximal at 60 μg·L -1 , at which it prevented the elevation in [Ca 2+ ] i. CONCLUSION: NGF protect cortical neurons against glutamate induced toxicity via “stabilizing” [Ca 2+ ] i level or suppression of the rise in [Ca 2+ ] i.

关 键 词：钙 大脑皮质 谷氨酸 神经生长因子 神经元 毒性 

分 类 号：R964[医药卫生—药理学] R965[医药卫生—药学]