卡托普利和依那普利拉对分离的大鼠心肌细胞内游离Ca^（2＋）浓度的影响  

Effects of captopril and enalaprilat on intracellular Ca 2+ content in isolated cardiomyocytes from rats 1

作　　者：王军[1] 章鲁[1] 齐建华[1] 张佩瑾[1] 魏丕敬[1] 顾培坤[1] 金正均[1] 

机构地区：[1]上海第二医科大学药理教研室 医用计算机教研室 电生理教研室

出　　处：《中国药理学报》1996年第3期233-235,共3页Acta Pharmacologica Sinica

摘　　要：目的：研究转换酶抑制剂卡托普利（Ｃａｐ）和依那普利拉（Ｅｎａ）对ＳＨＲ和ＷＫＹ大鼠心肌细胞内游离Ｃａ２＋浓度的影响．方法：用荧光探针Ｆｕｒａ２ＡＭ结合计算机图象处理技术测定分离心肌细胞内游离Ｃａ２＋浓度．结果：ＳＨＲ心肌细胞内游离Ｃａ２＋浓度（１７４±５ｎｍｏｌ·Ｌ－１）较ＷＫＹ大鼠（１４８±１５ｎｍｏｌ·Ｌ－１）高（Ｐ＜００１）．Ｃａｐ和Ｅｎａ能明显降低ＳＨＲ心肌细胞内游离Ｃａ２＋浓度（分别为１６１±１１和１６６±７ｎｍｏｌ·Ｌ－１，Ｐ分别＜００５），但对ＷＫＹ的无影响（Ｐ＞００５）．两药均能明显降低ＮＥ和ＡｎｇＩ引起的ＳＨＲ和ＷＫＹ大鼠心肌细胞内Ｃａ２＋升高，同时也能明显降低ＫＣｌ引起的ＳＨＲ细胞内Ｃａ２＋升高（Ｐ＜００５），但对ＷＫＹ大鼠的无明显影响（Ｐ＞００５）．结论：Ｃａｐ和Ｅｎａ对病理性电压依赖性Ｃａ２＋通道有直接抑制作用．IM: To study the effects of ACEI captopril (Cap) and enalaprilat (Ena) on intracellular Ca 2+ concentration ([Ca 2+ ] i) in cardiac myocytes isolated from SHR and WKY rats. METHODS: Using fluorescent probe Fura 2 AM combined with computer image processing technique to measure [Ca 2+ ] i. RESULTS: Resting [Ca 2+ ] i was higher in SHR cardiac myocytes (174±5 nmol·L -1 ) than that in WKY rat myocytes (148±15 nmol·L -1 , P <0 01). Cap and Ena decreased the resting [Ca 2+ ] i in SHR myocytes (161±11 and 166±7 nmol·L -1 , respectively, P <0 05) but not in WKY rat myocytes ( P >0 05). Both drugs inhibited [Ca 2+ ] i increment induced by KCl, NE, or Ang II in SHR and WKY rat myocytes except on KCl induced [Ca 2+ ] i increment in WKY rat myocytes ( P >0 05). CONCLUSION: Cap and Ena had direct effects on pathological voltage operated calcium channel in cardiac myocytes.

关 键 词：FURA-2 心肌 卡托普利 依那普利拉 钙 

分 类 号：R965.1[医药卫生—药理学] R972[医药卫生—药学]