热聚合法制备二苯甲酰-L-酒石酸手性分子印迹聚合物 Ⅰ.热聚合条件的优化  

Molecular Imprinted Polymers of Dibenzoyl-L-Tartaric Acid with Recognition Properties Prepared by Thermal Polymerization Method Ⅰ.Optimizing the Thermal Polymerization Conditions

作　　者：杨座国[1] 许振良[1] 

机构地区：[1]华东理工大学化学工程研究所,上海200237

出　　处：《华东理工大学学报（自然科学版）》2005年第5期580-584,611,共6页Journal of East China University of Science and Technology

基　　金：国家重点基础研究发展计划(973计划)资助(2003CB615705)

摘　　要：以二苯甲酰-L-酒石酸(L-DBTA)为模板分子,甲基丙烯酸甲酯(MMA)等为功能单体,乙二醇二甲基丙烯酸酯(GDMA)为交联剂,采用热聚合方法合成了L-DBTA手性分子印迹聚合物(ML-DBTA),讨论了不同功能单体、功能单体的用量、交联剂的用量、聚合温度、聚合时间、溶剂等对ML-DBTA合成的影响。通过ML-DBTA对底物的结合实验和高效液相色谱分析,合成的ML-DBTA对模板分子L-DBTA具有很好的识别性,对L-DBTA的选择性比二苯甲酰-D-酒石酸(D-DBTA)高,其分离因子(α)可达2.79。优化的热聚合条件为:n(L-DBTA)∶n(MMA)∶n(GDMA)=1∶4∶20,聚合温度60°C,聚合时间48 h,溶剂为乙腈。Using a functional monomer （methyl methacrylate, acrylamide, ethyl acrylate and acrylic acid） and a crosslinker （glycol dimethyacrylate）, molecularly imprinted polymers （MIPs） of dibenzoyl-L-tartaric acid （L-DBTA） with recognition properties were prepared by thermal polymerization method in the presence of a template （L-DBTA） in acetonitrile solution. The effects of different functional monomers, concentrations of functional monomers, cross-linker concentrations, polymerization temperatures, polymerization times, different solvents on the MIPs＇ recognition properties were discussed. The binding experiments and high-performance liquid chromatographic （HPLC） analysis of molecularly imprinted polymers （MIPs） of L-DBTA with recognition properties were conducted to evaluate the influences of various factors on DBTA binding. The experimental results indicate that the MIPs has higher L-DBTA binding ability than D-DBTA＇s and also higher than corresponding non-imprinted polymers. The binding ability is the highest at template （L-DBTA）. functional monomer（MMA）：crosslinker （GDMA）1：4：20 （molar ratio） as polymerization temperature is 60℃ and polymerization time is 48 h. Based on the optimal experimental results, the highest separation factor （a） of L-DBTA/D-DBTA of the MIPs can be 2.79.

关 键 词：分子印迹聚合物 二苯甲酰-L-酒石酸 热聚合 手性识别 制备 

分 类 号：O631[理学—高分子化学] TQ028[理学—化学]