注射用双黄连对人巨细胞病毒感染致肝损伤模型小鼠的保护作用研究  

Study on the Protective Effect of Shuanghuanglian Sterilized Powder for Injection on Hepatic Injury of Model Mice Induced by Human Cytomegalovirus Infection

作　　者：吴建军[1] 刘贵育[1] 陈贵海[1] 侯舒[1] 王明丽[1] 

机构地区：[1]安徽医科大学微生物学教研室,合肥市230032

出　　处：《中国药房》2005年第20期1536-1538,共3页China Pharmacy

基　　金：安徽省生物医药重大科技专项(01303003);教育部科学技术重点项目(01052);安徽省自然科学基金资助项目(2003043708)

摘　　要：目的:研究注射用双黄连对人巨细胞病毒(HCMV)感染致肝损伤模型小鼠的保护作用。方法:随机将小鼠分为正常对照组、感染模型组、更昔洛韦组和注射用双黄连(高、中、低剂量)组,除正常对照组外,其余各组均接种HCMV5d建立肝损伤模型,分别给予不同药物;10d后各组取眼眶血测定ALT、AST酶活性;颈椎脱臼处死小鼠,取肝脏评价其坏死程度并进行病理学观察。结果:正常对照组ALT、AST活性正常,肝脏未见异常改变;与正常对照组比较,感染模型组血清ALT、AST活性显著升高,肝脏的病理损伤明显;与感染模型组比较,注射用双黄连高、中剂量组肝脏ALT、AST活性显著降低,病理损伤明显减轻,而低剂量组血清ALT、AST活性与感染模型组比较无显著性差异;与感染模型组比较,更昔洛韦组ALT、AST活性显著降低;注射用双黄连高、中剂量组ALT、AST活性与更昔洛韦组比较无显著性差异。结论:注射用双黄连对HCMV感染致肝损伤模型小鼠具有保护作用。OBJECTIVE： To study the protective effect of shuanghuanglian sterilized powder for injection on hepatic injury of model mouse induced by human cytomcgalovirus（HCMV） infection. METHODS： The mice were randomized into norreal control group, infection model group, ganciclovir group and shuanghuanglian sterilized powder for injection group（high, middle and low dosage）, all of which except the normal control were vaccinated with HCMV 5d to establish the hepatic injury model, each group was administered with different drugs; 10 days later, the enzyme activity of ALT and AST in the eye sockets blood of each group was determined; The mice were put to death by cervical vertebra dislocation and the necrosis degree of livers from the dead mice was evaluated and the pathological observation was conducted. RESULTS： The activity of ALT and AST were normal in the normal control, and no significant changes were found in the livers of this group; Compared with the normal control, the plasma ALT and AST levels have been increased significantly and there was a significant pathological hepatic injury in the infection model group; Compared with the infection model group, significant decreases were found in the activity of ALT and AST in the high and middle dosage groups of shuanghuanglian sterilized powder for injection, and there were obvious alleviated pathologic hepatic injury while no significant differences were found in the plasma ALT and AST activity in the low dosage group; Compared with the infection model group, the ALT and AST activity of the ganciclovir group were lowed significantly;Compared with the ganciclovir group, no significant differences were found in the ALT and AST activity in the high and middle dosage groups of shuanghuanglian sterilized powder for injection. CONCLUSION： Shuanghuanglian sterilized powder for injection does have protective effect on hepatic injury of model mouse induced by HCMV.

关 键 词：人巨细胞病毒 注射用双黄连 小鼠 肝损伤 

分 类 号：R285.5[医药卫生—中药学]