紫外分光光度法测定O/W型紫草脂肪乳剂中总萘醌的含量  

Content Determination of the Total Naphthoquinone in Oil/Water Emulsion of Shikonin by Ultraviolet Spectrophotometry

作　　者：范芳芳[1] 刘泽蒲[1] 陈文[1] 江发寿[1] 

机构地区：[1]新疆石河子大学药学院,石河子市832002

出　　处：《中国药房》2005年第20期1577-1578,共2页China Pharmacy

摘　　要：目的:建立以紫外分光光度法测定O/W型紫草脂肪乳剂中总萘醌含量的方法.方法:总萘醌的含量以左旋紫草素计,溶剂为甲醇,检测波长为516nm.结果:左旋紫草素检测浓度在8.32～41.60μg/ml范围内与吸收度呈良好的线性关系(r=1.0000,n=5),平均回收率为98.6%(RSD=0.53%).结论:本方法简便、准确,回收率与精密度高,重现性好,可用于O/W型紫草脂肪乳剂中总萘醌含量的测定.OBJECTIVE： To establish a method for content determination of the total naphthoquinonc in oil/water emulsion of shikonin by ultraviolet spcctrophotomctry.METHODS： The content of the total naphlhoquinone was counted in L-shikonin,mcthanol was used as solvent and the wavelength was set at 516nm.RESULTS：The concentration of L shikonin had good linear correlation with absorbancc in the range of 8.32～41.60μg/ ml（r=1.0000, n= 5）, the average recovery was 98.6% （RSD= 0.53% ） . CONCLUSIONS： The present method is siraplc, precise and repllcablc resulting in high recovery and accuracy,it can be used to determine the content of total naphthoouinone in oil/wator cmulsion of shikonin.

关 键 词：O/W型 紫草脂肪乳剂 左旋紫草素 总萘醌 含量测定 

分 类 号：R927.2[医药卫生—药学]