复方丹参片与滴丸中丹酚酸B和丹参酮Ⅱ_A含量的比较研究  

Comparative Study on the Contents of Salvianolic Acid B and TanshinoneⅡ_ A in Compound Danshen Tablets and Its Drop Pills

作　　者：陈日来 李玉珍[2] 陈晓凯[2] 徐玉红[2] 路伟[2] 王蔓琳[2] 

机构地区：[1]深圳市福田区梅林医院,深圳市518049 [2]广东医学院附属福田医院,深圳市518033

出　　处：《中国药房》2005年第20期1595-1597,共3页China Pharmacy

摘　　要：目的:测定并比较复方丹参片与滴丸中丹酚酸B和丹参酮ⅡA的含量。方法:色谱柱为EclipseXDBC18,丹酚酸B、丹参酮ⅡA流动相分别为甲醇-0·2%磷酸溶液(40∶60)、甲醇-水(85∶15),流速为1·0ml/min,柱温为30℃,检测波长为280nm。结果:丹酚酸B和丹参酮ⅡA进样量均在0·1μg^2·0μg范围内与峰面积值呈良好线性关系(r丹酚酸B=0·9998、r丹参酮ⅡA=0·9999);复方丹参片中丹酚酸B、丹参酮ⅡA的平均回收率分别为100·7%(RSD=1·9%)、101·0%(RSD=1·5%),复方丹参滴丸中丹酚酸B、丹参酮ⅡA的平均回收率分别为98·6%(RSD=1·2%)、98·8%(RSD=1·4%)。结论:本方法准确、可靠、重现性好;复方丹参片中丹酚酸B和丹参酮ⅡA含量较高,复方丹参滴丸中则未定量检出上述2成分。OBJECTIVE： To determine and to compare the content of salvianolic acid B and tanshinone ⅡA in compound danshen tablets and its drop pills.METHODS：The determination was performed on Eclipse XDB Cla with column temperature at 300℃, the mobile phases were methanol- 0.2% phosphoric acid solution（40 ： 60） and methanol- water （85 ： 15）, respectively with the flow rate at 1.0ml/ min and the detective wavelength at 280nm.RESULTS： Good linear relations were obtained when the sample size of salvianolic acid B and tanshinone ⅡA were within the range of 0.1μg～2.0μg（ r = 0.9 998 for salvianolic acid B and r = 0.9 999 for tanshinone ⅡA）; The average recoveries of salvianolic acid B and tanshinone ⅡA of compound danshen tablets were 100.7% （RSD= 1.9%） and 101.0% （RSD = 1.5%）, respectively; And those for the compound danshen drop pills were 98.6% （RSD = 1.2%） and 98.8% （RSD 1.4%）,respectively.CONCLUSION：The method was accurate,reliable and reproducible .The contents of salvianolic acid B and tanshinone ⅡA in compound tanshen tablets are higher, while no fixed quantity were detected in the above 2 components in compound danshen drop pills.

关 键 词：高效液相色谱法 复方丹参片 复方丹参滴丸 丹酚酸B 丹参酮ⅡA 含量 

分 类 号：R927.2[医药卫生—药学]