酶体外定向进化(Ⅲ)展示技术及其应用  

Directed Evolution of Enzymes in Vitro(Ⅲ) Display Technology and Its Application

作　　者：夏启容[1] 方柏山[1] 洪燕[1] 

机构地区：[1]华侨大学材料科学与工程学院,福建泉州362021

出　　处：《华侨大学学报（自然科学版）》2005年第4期333-337,共5页Journal of Huaqiao University(Natural Science)

基　　金：国家自然科学基金资助项目(20276026;20446004);福建省科技计划重点基金资助项目(2003I020)

摘　　要：利用展示技术构建的蛋白质/多肽的突变体库,可以通过高通量进行高效地分析和筛选,因此特别适用于改造蛋白质.文中综述噬菌体、细胞表面、核糖体、mRNA等展示技术的原理和方法,以及其在酶定向进化中的应用.With the ability of conveniently assaying and analyzing a large number of mutated proteins in a high-throughput way, display systems of libraries of mutated proteins or peptides on phage or some biological resources have been used to improve proteins and enzymes. In this article, we review recent studies on display technology, which include not only the principles and methods of phage display, cell-surface display, ribosome display and mRNA display, but also their application in aspect of directed evolution of enzymes.

关 键 词：展示技术 酶 定向进化 噬菌体 细胞表面 核糖体 MRNA 

分 类 号：Q349.5[生物学—遗传学] Q785