益妇宁软胶囊对去卵巢大鼠雌激素受体及一氧化氮水平的影响  

Effect of the Yifuning soft gelatin capsules on Estrogen Receptor and Nitric oxide levels in ovariectomized rats

作　　者：肖炜[1] 马云[2] 邓虹珠[3] 魏连波[1] 

机构地区：[1]南方医科大学珠江医院,广东广州510282 [2]南方医科大学南方医院,广东广州510515 [3]南方医科大学中医系,广东广州510515

出　　处：《中国中药杂志》2005年第20期1598-1602,共5页China Journal of Chinese Materia Medica

基　　金：军队医药卫生科研基金课题(2000B01);广东省中医药局项目(400001)

摘　　要：目的:观察益妇宁软胶囊(YFN)对去卵巢大鼠雌激素受体(ER)、一氧化氮(NO)和一氧化氮合酶(NOS)水平的影响。方法:50只SD雌性大鼠随机分为5组:正常对照组、模型对照组、已烯雌酚片组(DT组)、YFN大、小剂量组。给药4周后用放射免疫法测定大鼠血清雌二醇(E2)水平,放射配体结合分析法测定子宫和主动脉ER数目,光镜下观察子宫组织病理学变化,同时测定主动脉NO,NOS水平和子宫指数。结果:YFN能明显升高大鼠去卵巢后降低的血清E2水平、子宫指数及ER含量(P<0.05或P<0.01),改善子宫组织病理变化;提高主动脉ER含量及NO,NOS水平(P<0.01或P<0.05)。结论:YFN可以治疗更年期综合征,同时防治心血管疾病的发生。Objective： To investigate the effects of the Yifiming soft gelatin capsules（YFN）on estrogen receptor （ER）and nitric oxide （NO） levels in ovariectomized rats. Method： Fifty female mature sprague-dawley rats were randomly divided into 5 groups：normal control; model control; diethylstilbestrol ablets（DT）; YFN （high dose and low dose）. After 4 weeks＇ treatment, the serum E2 levels were detected by radioimmunoassay. The estrogen receptor（ ER） levels of uterus and artery were detected with method of radioligand binding assay of receptor （RBAR）. The uterus pathologic changes were investigated with light microscope. NO and NOS levels of the artery and the uterus index were detected too. Result： YFN can obviously improve serum E2, increase index and ER of uterus （ P 〈0.01 or P 〈0.05）, and ameliorate the uterus＇ pathologic changes in OVX rats. It also can increase the artery＇ ER, NO and NOS levels. Conclusion： YFN can cure the climacteric syndrome and prevent the cardiovascular disease after post-menopause.

关 键 词：益妇宁软胶囊 去卵巢大鼠 一氧化氮 雌激素受体 

分 类 号：R285.5[医药卫生—中药学]