全反式维甲酸合钇(Ⅲ)配合物对DNA的切割和键合作用(英文)  

DNA Binding and Cleavage Activity of Y(Ⅲ) Complex with All-trans Retinoic Acid

作　　者：宋玉民[1] 宋小利[1] 栾尼娜[1] 杨培菊[1] 芦小林[1] 王流芳[2] 刘颖梅[2] 

机构地区：[1]西北师范大学化学化工学院,兰州730070 [2]兰州大学应用有机国家重点实验室,兰州730000

出　　处：《无机化学学报》2005年第11期1661-1666,共6页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.50272027);甘肃省自然科学基金(No.3ZS051-A25-021);甘肃省高分子材料重点实验室西北师范大学A级重点学科资助课题。

摘　　要：以紫外光谱、荧光光谱、粘度法和凝胶电泳方法研究了全反式维甲酸合钇!配合物与DNA的作用。结果表明,该配合物能在生理条件下比配体和金属离子更有效地切割质粒DNA,体系离子强度和pH值的变化对配合物的切割活性有较大影响,自由基捕捉剂的加入不影响配合物的切割活性。该配合物对DNA的切割可能通过水解机理进行。该配合物可使DNA的粘度增加,使EB-DNA体系的荧光强度和DNA溶液的紫外吸收强度降低。据此推断,该配合物主要以嵌入方式与DNA作用。The interaction of the Y（Ⅲ） complex of all-trans retinoic acid （Y（RA）R·Ac·8H2O） with DNA has been characterized by UV-absorption and fluorescence spectroscopy, viscosity measurements and agarose gel electrophoresis method. The results indicate that the Y（RA）2·Ac·8H2O can more effectively promote the cleavage of plasmid DNA than that of all-trans retinoic acid and Y^3＋ at physiological pH and temperature, which may be one of the reasons why the inhibitory effect of Y（RA）2·Ac·8H2O on human bladder line EJ cells is much greater than that of retinoic acid. It was found that the process of plasmid DNA cleavage was sensitive to ionic strength and pH, however, these radical scavengers almost had no effect on the DNA cleavage reaction. The above results suggest that the cleavage of DNA by Y（RA）2·Ac·8H2O does not produce diffusible hydroxyl radicals via the Fenton reaction. The results of viscosity measurements, UV-absorption and fluorescence characterization on the interaction of Y（RA）2·Ac·8H2O with Calf thymus DNA show that the Y（RA）2·Ac·8H2O binds to DNA mainly in an intercalating mode.

关 键 词：全反式维甲酸合钇(Ⅲ) 切割反应 质粒DNA(pBR322-DNA) 小牛胸腺DNA 

分 类 号：O614.33[理学—无机化学]