E-test和纸片扩散法检测氟康唑和两性霉素B体外抗念珠菌活性  

In vitro susceptibilities to fluconazole and amphotericin B against Candida spp. determined by E - test and disk diffusion methods

作　　者：吴伟元 何林 吴劲松 李文青 程锦娥 宿建 

机构地区：[1]深圳市人民医院检验医学部,广东深圳518020

出　　处：《中国临床药理学杂志》2005年第5期378-380,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的 比较E—test和纸片扩散法检测氟康唑和两性霉素B对念珠菌的 体外抗菌活性。方法用E—test和纸片扩散法检测123株念珠菌对氟康唑和 两性霉素B的敏感性。结果E—test法和纸片扩散法检测71株白色念珠菌 和30株热带念珠菌对氟康唑／两性霉素B敏感性,2种方法的一致性分别为 94．4％(67／71)／100％(71／71)和100％(30／30)／86．7％(26／30)。结论2种 方法均可检测氟康唑和两性霉素B对白色念珠菌和热带念珠菌的体外抗菌活 性。Objective To investigate the correlation between E -test and disk diffusion （DD） methods used to detect the antifungal activitiy of fluconazole and amphotericin B against Candida spp.. Methods The E - test and DD methods were performed to detect the susceptibilities of fluconazole and amphotericin B against 123 isolates of Candida spp.. Results For C. albicans and C. tropicalis, the coincidences between E -test and DD methods applied to detect the susceptibilities fluconazole/amphotericin B, were 94.4% （67/71）/100% （71/71） and 100% （30/30）/86.7% （26/ 30）, respectively. Conclusion The DD method can be performed to detect the susceptibilities of fluconazole and amphotericin B against C. albicans and C. tropicalis in clinical laboratory.

关 键 词：念珠菌 耐药 氟康唑 两性霉素B 

分 类 号：R978.5[医药卫生—药品] R969.3[医药卫生—药学]