新抗生素万隆霉素与金属离子的相互作用  

Interaction Between a New Antibiotic Wanlongmycin and Metal Ions

作　　者：高向阳[1] 林壁润[2] 姚汝华[1] 陈念[3] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510640 [2]广东省农科院植物保护研究所,广东广州510640 [3]华南农业大学生命科学学院,广东广州510642

出　　处：《华南理工大学学报（自然科学版）》2005年第10期84-89,共6页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家"863"高科技计划资助项目(2001AA-246015)国家自然科学基金资助项目(30471161)广东省自然科学基金团队资助项目(2KB042051)广东省自 然科学基金资助项目(032011)

摘　　要：对万隆霉素的紫外和荧光光谱进行了研究,发现万隆霉素的乙醇溶液在190- 300nm的范围内出现较强的紫外吸收,最高吸收峰的位置为202和228 nm.选择290和330 nm为荧光扫描的最适激发和发射波长对万隆霉素与金属离子之间的相互作用进行研究.结果表明:Ca2+,Al3+,Cu2+和Mg2+不仅能使万隆霉素的紫外吸收光谱发生减色效应和轻微的紫移,而且可使其荧光光谱发生显著的猝灭,说明万隆霉素与金属离子之间发生了相互作用并形成稳定的配合物;不同浓度的Zn2+对万隆霉素的紫外吸收、相对荧光强度及峰的位置几乎没有影响,说明万隆霉素与Zn2+之间不发生相互作用.The ultraviolet （UV） and fluorescent spectra of Wanlongmycin were first investigated, with a retauvely strong UV absorbance occurring within the range of 190 to 300nm in alcohol solvent, and two highest peaks located at the wavelengths of 202 nm and 228 nm. Then, the wavelengths of 290 nm and 330 nm were respectively selected as the optimum fluorescent excitation and emission wavelengths to study the interaction between Wanlongmycin and metal ions. The results indicate that, in the presence of Ca^2＋, Al^3＋, Cu^2＋ and Mg^2＋, a decrease in peak intensity and a violet shift of the UV spectrum of Wanlongmycin, as well as an obvious fluorescent quenching effect, can be observed, which means that Wanlongmycin can interact with these metal ions to form a stable Wanlongmycin-metal ion complex. Moreover, it is indicated that Zn^2＋ with different concentrations hardly influences the UV spectrum, the relative fluorescent intensity and the location of the peaks, which means that there is no interaction between Wanlongmycin and Zn^2＋.

关 键 词：万隆霉素 金属离子 紫外光谱 荧光光谱 

分 类 号：TQ455.5[化学工程—农药化工] R978.16[医药卫生—药品]