吉西他滨诱导胰腺癌细胞株SW1990的耐药作用与硫氧还蛋白还原酶活性的改变  

Drug Resistance and Activity Changes of Thioredoxin Reductase in Pancreatic Cancer Cell Strain SW1990 Induced by Gemcitabine

作　　者：牛备战[1] 陈革[1] 李丽君[1] 吴元德[1] 赵玉沛[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院基本外科,北京100730

出　　处：《中国医学科学院学报》2005年第5期606-610,T0002,共6页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(30371389);北京市自然科学基金(7032038)资助~~

摘　　要：目的建立对吉西他宾耐药的胰腺癌细胞株,探讨硫氧还蛋白还原酶(TrxR)在耐药过程中的作用。方法采用间歇浓度递增法诱导胰腺癌细胞株SW1990对吉西他宾耐药,检测其生物学性状变化,并对比观察TrxR活性变化。结果药物培养9个月后,得到稳定传代的SW1990/GZ耐药细胞株;其形态学、生长特征等均发生了明显变化,细胞变圆、体积缩小、内质网扩张、颗粒样物质增多及细胞倍增时间延长;对吉西他滨、5-氟尿嘧啶、阿霉素和丝裂霉素的耐药性均显著增加;耐药细胞的TrxR活性也明显增高。结论SW1990/GZ有多药耐药现象,TrxR在耐药过程中起一定作用。Objective To establish gemcitabine-resistant pancreatic cancer cell strain and study the role of thioredoxin reductase （TrxR） in drug-resistant process. Methods Gemcitabine-resistant pancreatic cancer cell strain SW1990/GZ was induced by increasing drug dosage intermittently, then the changes of its biological features and the activity of TrxR were examined. Results Stable drugresistant SW1990/GZ cell strain was established by culturing with gemcitabine for 9 months. The morphology and growth characteristics of the cell strain changed remarkably, The cells shrinked and became rounder; its endoplasm expanded; granular substances increased; and the doubling-time was prolonged. Resistance of the cell line to gemcitabine, fluorouracil, adriamycin, and mitomycin significandy increased. The TrxR activity of the drug-resistant cells was increased markedly. Conclusion SW1990/GZ has certain multidrug resistance to some chemotherapy drugs, and TrxR plays a role in the drug-resistant process.

关 键 词：胰腺癌 多药耐药 吉西他宾 硫氧还蛋白还原酶 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] R735.9[医药卫生—基础医学]