环维黄杨星D对离体豚鼠胆道括约肌收缩力的影响  

Effect of Cyclovirobuxine D(CVB-D) on Oddi's sphincter contraction

作　　者：李蕾[1] 章蕴毅[1] 

机构地区：[1]复旦大学药学院药理教研室,上海200032

出　　处：《中成药》2005年第10期1178-1181,共4页Chinese Traditional Patent Medicine

摘　　要：目的:研究环维黄杨星D(CyclovirobuxineD,CVBD)对离体豚鼠胆道括约肌的收缩作用。方法:1)利用离体豚鼠胆道括约肌,加入不同浓度(1×10-7M～3×10-6M)CVBD,观察由KCl或Ach引起的括约肌快速收缩相和持续相的变化。2)加入维拉帕米(Ver)作用后,再加入CVBD,观察二者合用对KCl或Ach引起的括约肌收缩变化。结果:对KCl引起的标本收缩,CVBD使快速相峰值显著下降,且有量效关系,而持续相呈轻度升高,坪/峰比值也呈剂量效应的增加。但对Ach引起的收缩,CVBD使快速相、持续相、坪/峰值均显著下降,且有一定量效关系。Verapamil合用CVBD对KCl引起的快速收缩相峰值下降,但坪值则与单用Ver相当。对Ach引起的快速相收缩,二者也有相似的协同作用,但此时坪值几乎消失。结论:CVBD对括约肌的收缩作用与引起收缩的刺激剂有关,也与收缩时相有关,反映了CVBD对不同钙离子通道的影响。AIM： To study the effect of CVB-D on contraction of guinea pig Oddi＇s sphincter in vitro. METHODS： Within the range from 1 × 10^-7M to 3 × 10^-6M, CVB-D＇s effects on the smooth muscle under the following conditions were observed： 1 ） the two phases of the Oddi＇s sphincter contractive curve caused by 80 mM K^＋ and 5 × 10^-6M Ach; 2）together with verapamil, the contraction of Oddi＇s sphincter induced by 80 mM K^＋ and 5 × 10^-6 M Ach respectively. RESULTS： The relation between the amount of CVB-D and rapid contraction of desived inihibitory response was possessed of a dose-effect one, as well as ratio of plateau and peak of contractions, and slightly elevated contraction in continuous phase. CVB-D kept rapid phase peak value and plateau/peak value decreased markedly that expressed a dose-effect relationship. Contrary to CVB-D. Ach caused rapid, continuous phase and plateau/peak value to fall according as CVB-D decreased. Combination of verapamin and CVB-D led rapid phase peak value induced by KCl to lessen, but do not influence verapamin plateau used only and vanish plateau of rapid phase induced by Ach. CONCLUSION： CVB-D＇s effects on the contraction of Oddi＇s sphincter are related to the agonists and contractive phases, which reflect its effects on Ca^2＋ channels.

关 键 词：环维黄杨星D 维拉帕米 胆道括约肌 钙离子通道 

分 类 号：R285.5[医药卫生—中药学]