光谱法研究2-(4-二甲氨基苯基)-5-氟-6-吗啉-1-氢-苯并咪唑与小牛胸腺DNA的相互作用  

Spectroscopic Research on the Interaction of 2-(4-Dimethylaminophenyl)-5-Fluoro-6-(Morpholin-4-yl)-1H-Benzimidazole and Calf Thymus DNA

作　　者：唐凌天[1] 王毅[1] 刘新起[1] 胡少文[1] 褚泰伟[1] 王祥云[1] 

机构地区：[1]北京大学化学与分子工程学院应用化学系,北京100871

出　　处：《光谱学与光谱分析》2005年第10期1618-1621,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(20171005)资助项目

摘　　要：研究了2-(4-二甲氨基苯基)-5-氟-6-吗啉-1-氢-苯并咪唑(1)在不同pH条件下的紫外-可见吸收光谱,采用非线性最小二乘法得出分子1的三级加质子常数lgβ1,lgβ2,lgβ3分别为4.96±0.03,5.72±0.07和7.95±0.10.当pH 3.40时,分子1主要以一价离子状态存在,紫外-可见吸收光谱及荧光光谱表明该条件下分子与小牛胸腺DNA存在明显的相互作用,并得出分子1与DNA的结合常数Kb为(2.30±0.10)×104mol-1·L.当分子浓度为10-8～1.2×10-6mol·L-1时,荧光强度随DNA含量的增加而线性增强,分子1是一种潜在的测定DNA的定量试剂.UV-Vis spectra of the aqueous solutions of 2-（4-dimethylaminophenyl）-5-fluoro-6-（morpholin-4-yl）-1H-benzimidazole （1） at different pH values revealed that compound 1 is a tertiary base, which can combine three protons. Through the non-linear least square method, the logarithm of the three accumulative protonation constants of compound 1, namely, 1gβ1, 1gβ2 and 1gβ3, were determined to be 4.96±0.03, 5.72±0.07 and 7.95±0.10, respectively. UV-Vis and the steady-state fluorescence spectra indicated that a special interaction occurs between compound 1 and calf thymus DNA at the pH value of 3.40, of which the binding constant, Kb, is （2.30 ± 0.10） × 10^4 mol^-1·L. Compound 1 in the concentration range of 10^-8 to 1.2 × 10^-6 mol·L^-1 has a potential application to the quantitative determination of DNA.

关 键 词：2-(4-二甲氨基苯基)-5-氟-6-吗啉-1-氢-苯并咪唑 小牛胸腺DNA 紫外-可见吸收光谱 荧光光谱 

分 类 号：O657.3[理学—分析化学]