美兰对变形链球菌及菌斑抑制的实验研究  

Inhibition Effect of Methylene Blue on the Acids Production of Streptococcus mutans

作　　者：王成龙[1] 刘洪臣[1] 李振钢[2] 沈世杰[3] 储冰峰[1] 赵皿 黄力子 

机构地区：[1]中国人民解放军总医院口腔科 [2]中国人民解放军总医院医疗处,北京100853 [3]中国人民解放军军事医学科学院仪器中心,北京100850 [4]第四军医大学口腔医院口腔内科,陕西西安710032

出　　处：《华西口腔医学杂志》2005年第5期407-409,共3页West China Journal of Stomatology

摘　　要：目的研究美兰对变形链球菌生长和产酸代谢的作用以及对体外菌斑糖酵解模型产酸代谢的作用,以探讨美兰防龋的可行性。方法采用比浊法测定不同培养条件下变形链球菌培养液的OD值;采用气相色谱法检测不同培养条件下变形链球菌培养液中有机酸的种类和数量;测定不同处理条件下体外菌斑糖酵解模型产酸液的pH值。结果(1)美兰组细菌OD值低于生理盐水组,二者之间的差异具有统计学意义;(2)不同血清型变形链球菌的3个处理组中均可检测到有机酸的产生,葡萄糖组有机酸的总量最多,美兰组有机酸的总量最少;(3)美兰组产酸液的pH值与阴性对照组之间的差异有统计学意义,与阳性对照组之间的差异无统计学意义。结论美兰可以抑制变形链球菌的生长、产酸代谢以及菌斑的产酸代谢。Objective To study the effect of methylene blue on the growth and acid production of Streptococcus nuaans, and the effect of methylene blue on acid production metabolism in plaque glyeolysis model （i-PGM） in vitro, and investigate the practicability of methylene blue as a new kind of dental caries prevention agent. Methods Nephelometer method was used to measure OD value of Streptococcus mutans culture fluid in the different incubation conditions. The kinds and quantifies of acid produced by Streptococcus mutans in the different incubation conditions were measured with gas chromatography, pH values of glyeolysis buffer media of i-PGM in the different treatment conditions were measured by ORION electrode. Results ①The OD value of Streptococcus mutans treated by methylene blue was lower than that by normal saline, and there was significant statistical difference between them. ②The kinds of acid in three different culture fluid were same, but the total quantities of acid were significantly different among three different culture fluid, in which the total quantities of acid of culture fluids treated by glucose was the greatest, and treated by methylene blue was the lest. ③The pH value of i-PGM treated by methylene blue was significantly different compared with negative control group, but was not significantly different compared with positive control group. Conclusion Methylene blue can inhibit the growth and acid production metabolism of Streptococcus mutans and acid production metabolism of i-PGM.

关 键 词：美兰 链球菌 变异 生长 产酸 龋病预防 

分 类 号：R96[医药卫生—药理学]