三种基因转录因子在大鼠耳蜗大上皮嵴的表达  

Expression of three kinds of transcription factors in greater epithelial ridge cells of rat cochlear

作　　者：张媛[1] 胡吟燕[1] 宋巍[1] 郭维维[1] 翟所强[1] 

机构地区：[1]解放军总医院耳鼻咽喉科研究所,北京100853

出　　处：《中华耳鼻咽喉头颈外科杂志》2005年第9期664-666,共3页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：海外青年学者合作研究基金项目(30228029);国家自然科学基金项目(30440063);北京市自然科学基金项目(7053076)

摘　　要：目的检测Math1、Hes1及Hes5在大鼠耳蜗大上皮嵴(greaterepithelial ridge,GER)的表达,初步探讨各基因对毛细胞分化和再生的作用。方法分别取出生当天(P0),出生后第1天(P1)、第3天(P3)、第4天(P4)和第5天(P5)大鼠耳蜗,利用机械分离和酶消化相结合的方法分离出相对纯化的GER细胞。Trizol法提取GER细胞总RNA,逆转录聚合酶链反应检测Math1、Hes1及Hes5在GER的表达,琼脂糖凝胶电泳检测结果。结果Math1在P0～P5大鼠GER均有表达,Hes1只在P0～P3大鼠GER有表达,而Hes5在P0～P5大鼠GER中均无表达。结论Math1的表达水平可能只有累积至一定量,才能诱导GER增殖、分化成毛细胞,并且此过程可能同时受Hes1的控制。Objective To detect the expression of Mathl, Hesl and Hess in greater epithelial ridge （GER） cells of rat cochlear and explore their influence on hair cell differentiation. Methods Postnatal day 0 （P0） , day 1 （ P1 ） , day 3 （ P3 ） day 4 （P4） and day 5 （ P5 ） rat cochlear were dissected respectively and then pure GER cells were separated by a combinatorial approach of attachment and mechanical separation. The total RNA of GER cells was extracted by Trizol one step method and the expression of Mathl, Hesl and Hess in GER cells was detected with reverse transcription polymerase chain reaction. Results Mathl was expressed in P0- P5 rat GER cells and Hesl was expressed only in P0 - P3 rat GER cells, while there was no expression of Hess in P0- P5 rat GER cells. Conclusions Probably only when the expression of Mathl reaches a certain level can it induce GER cells to differentiate into hair cells. Meanwhile this process might controlled by Hesl to some extent.

关 键 词：逆转录聚合酶链反应 转录因子 毛细胞 内 基因转录因子 大鼠 耳蜗 上皮 聚合酶链反应检测 琼脂糖凝胶电泳 细胞分化 

分 类 号：R764[医药卫生—耳鼻咽喉科]