中国帕米尔高原豆科植物根瘤菌多样性研究  

Diversity of Leguminous Rhizobia in Pamirs Plateau of China

作　　者：韦革宏[1] 曹鹏[1] 龚明福[1] 吕双庆[2] 苏鹏[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100 [2]塔里木大学植物科技学院,新疆阿拉尔843300

出　　处：《西北植物学报》2005年第9期1811-1815,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30470040);全国优秀博士学位论文作者专项基金(200254);教育部新世纪优秀人才支持计划基金(NCET-04-0953);西北农林科技大学拔尖人才支持计划基金

摘　　要：从新疆帕米尔高原采集豆科植物根瘤,经分离纯化获得68株供试根瘤菌,并选取36株参比菌株,进行唯一碳源利用、唯一氮源利用、对抗生素和染料抗性、耐盐性、初始pH生长、温度生长范围及石蕊牛奶反应、氧化酶、过氧化氢酶、脲酶共117项生理生化性状测定,并进行比较分析.结果表明:帕米尔高原豆科植物根瘤菌在碳氮源利用、抗生素敏感性、对染料抗性程度等方面存在着差异.该地区豆科植物根瘤菌均能耐受低温,并具有较强的耐盐、耐碱能力,所有供试菌株均能在初始pH 9～12的YMA培养基上生长,84.7%的菌株能耐受3.0%的NaCl.从数值分类树状图可见,在86%的相似水平上未知菌株构成了4个新的表观群.第Ⅰ、Ⅱ、Ⅲ、Ⅳ类群分别有19、5、6、8株菌,中心菌株分别为NWHQLP412、NWHQLP481、NWTX1901、NWTX952.68 strains of rhizobia were isolated from the leguminous nodules collected in Pamirs Plateau located in Xinjiang,and 118 physiological and biochemical characters of these strains and 36 known control strains such as uses of single carbon and nitrogen sources ,antibiotic and dye resistances ,salt and alkali tolerances,initial growth pH, growth temperature limits, litmus and milk responses, oxidase, peroxidase and urease were determined and compared. The results revealed that the leguminous rhizobia in Pamirs Plateau differed in uses of single carbon and nitrogen sources,antibiotic sensitivity,and dye resistance. All leguminous rhizobia were capable of tolerating low temperature and had high capabilities of salt and alkali toler- ances;all the strains under study could grew on YMA medium with its initial pH at 9～12 and 84.7% of them can tolerate 3. 0% NaC1. The dendrogram of numerical taxonomy shows that at the similarity of 86%,the unknown strains formed four new apparent clusters, clusters Ⅰ ,Ⅱ, Ⅲ and Ⅳ contained 19,5,6 and 8 strains ,respectively,and the central strains were NWHQLP412,NWHQLP481,NWTX1901 and NWTX952.

关 键 词：帕米尔高原 根瘤菌 多样性 

分 类 号：Q948.9[生物学—植物学] Q949.751.9