侯选抑瘤基因BRD7及家族蛋白的功能研究进展  

Advances in Function Analysis of a Candidate Tumor Suppressor Gene BRD7 and Its Family Members

作　　者：刘华英[1] 李桂源[1] 

机构地区：[1]中南大学肿瘤研究所,长沙410078

出　　处：《生物化学与生物物理进展》2005年第9期811-816,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金重大项目(30330560);国家自然科学基金资助项目(30200135;30300175;30400238).~~

摘　　要：溴区结构(bromodomain)是近年来发现的广泛分布于多种生物中的一种高度保守的结构域,溴区结构蛋白通过参与信号依赖性的基因转录调控而广泛参与细胞内重要的生命活动.BRD7基因是1999年克隆的一个新的bromodomain基因,GenBank登录号为AF152604或AF152605.eMotif分析表明,BRD7蛋白包含多个磷酸化位点和一个保守bromodomain功能域,Blast显示BRD7蛋白与人的Celtix-1及鼠的bromodomain蛋白BP75具有高度的同源性.利用转基因技术已证实,在鼻咽癌细胞系HNE1中过表达BRD7基因可以抑制其细胞生长和细胞周期G1-S的进程,并部分逆转鼻咽癌细胞HNE1的恶性表型.为了全面地揭示BRD7基因的细胞内生物学功能,深入了解BRD7基因的细胞内整体信息流向,中南大学肿瘤研究所细胞遗传室已从上、中、下游三个不同层面对BRD7基因的功能研究展开了初步的探索.Bromodomain is an evolutionally conserved domain and is identified in proteins strongly implicated in signal-dependent gene transcription regulation, BRDT, a novel bromodomain gene, has been cloned by cDNA RDA （cDNA Representational Difference Analysis） in 1999, The GenBank accession number is AF152604 or AF152605. eMotif analysis revealed that BRD7 protein contains a conserved bromodomain and several important phosphorylation sites, Multiple sequence alignment program showed high ratio identity between BRD7 protein, protein Celtix-1 and musculus bromodomain-containing protein BP75. Over expression of BRD7 in Nasopharyngeal carcinoma （NPC） cells can inhibit cell proliferation and cell cycle progression from G1 to S phase, and can partly inhibit the aberrant growth of NPC cells. In order to further address the signal pathway and the possible functions of BRD7 gene, function analysis of BRD7 gene was performed through three different cellular physiology levels including up- and down-stream, interplay issues of BRD7 gene.

关 键 词：BRD7基因 溴区结构功能域 BRD7基凼功能 

分 类 号：Q74[生物学—分子生物学]