咪喹莫特对人表皮朗格汉斯细胞及HaCaT细胞分泌细胞因子的影响  

Effects of Imiquimod on the Secretion of Cytokines by Human Epidermal Langerhans Cells and Ha- CaT Cells

作　　者：缪旭[1] 骆丹[1] 吉玺[1] 徐丽贤[1] 闵玮[1] 林向飞[1] 许阳[1] 

机构地区：[1]南京医科大学附属第一医院皮肤科,210029

出　　处：《中华皮肤科杂志》2005年第10期619-621,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金(30371294)

摘　　要：目的研究咪喹莫特对人表皮朗格汉斯细胞(LC)及HaCaT细胞细胞因子分泌水平的影响。方法联用密度梯度离心和免疫磁珠的方法分离纯化LC,以5μg/mL咪喹莫特刺激LC及HaCaT细胞,4h后取培养上清,用ELISA方法检测肿瘤坏死因子α、白介素1β及白介素6的含量。结果 LC咪喹莫特处理的实验组培养上清中肿瘤坏死因子α、白介素1β及白介素6含量均较未处理对照组明显增高(P均＜0．05)。HaCaT细胞3种细胞因子分泌量在实验组与对照组中差异无统计学意义(P均＞0．05)。结论咪喹莫特能增加LC细胞因子肿瘤坏死因子α、白介素1β及白介素6的分泌,但对HaCaT细胞上述3种细胞因子的分泌未见明显影响。Objective To investigate the ellects ol imiquimod on cytokine secrehons of normal human epidermal Langerhans cells （LCs） and HaCaT cells, and to better understand the mechanism of immune regulation by imiqulmod. Methods LCs were sorted from prepared epidermal cells by density gradient centrifugation and magnetic-activated cell sorting （MACS）. LCs and HaCaT cells were cultured in media with or without 5μg/mL of imlquimod for 4 hours, then cell-free culture supernatants were harvested, cytokines were detected by ELISA kitS. Results TNF-α, IL-1β and IL-6 secreted from imiquimod treated LCs were all higher than those from control LCs （all P 〈 0.05）, but there is no statistical difference between the cytokines from imiquimod treated HaCaT ceils and those from control HaCaT cells. Condusion Imiquimod can upregulate the secretions of cytokines from human epidermal LCs but not from HaCaT cells.

关 键 词：咪喹莫特 郎格尔汉斯细胞 角蛋白细胞 细胞因子类 HACAT细胞 分泌细胞因子 朗格汉斯细胞 人表皮 肿瘤坏死因子α 白介素1β 

分 类 号：R96[医药卫生—药理学]