天然抗角蛋白IgM调理巨噬细胞吞噬金黄色葡萄球菌的作用  

Influence of Natural Antikeratin IgM on Phagocytosis of Staphylococcus aureus by Phogocytes

作　　者：安金刚[1] 李巍[1] 付萌[1] 王刚[1] 李承新[1] 刘玉峰[1] 

机构地区：[1]第四军医大学西京医院皮肤科,西安710032

出　　处：《中华皮肤科杂志》2005年第10期625-627,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金重点项目(30330510);军队"十五"卫生科研基金(01Z086)

摘　　要：目的探讨天然抗角蛋白IgM对金黄色葡萄球菌的结合反应和介导调理吞噬的功能。方法ELISA法及间接免疫荧光法检测天然抗角蛋白IgM抗体3B4与金黄色葡萄球菌的结合。将3B4与金黄色葡萄球菌作用后与巨噬细胞共孵育,菌落形成实验及流式细胞仪分析3B4介导细菌的调理吞噬作用。结果 ELISA与间接免疫荧光均发现3B4可以与细菌结合,菌落形成实验显示3B4作用组菌落数量显著少于阴性对照组,流式细胞仪分析显示巨噬细胞对3B4作用后细菌的吞噬作用明显增强。结论天然抗角蛋白IgM抗体3B4可以结合并介导调理吞噬金黄色葡萄球菌,提示天然IgM在抗细菌天然免疫中具有一定的作用。Objectives To investigate the binding of natural lgM to Stapohylococcus aureus and its role in the phagoc, ytosis of S. aureus by phagocytes, and to pave way for further study on the role and mechanism of natural lgM in defense of bacteria. Methods The binding of natural antikeratin IgM 3B4 to S. aureus was analyzed by ELISA and indirect immunofluorescence. The role of 3B4 in the phagocytosis was analyzed by colony forming assay and flow cvtometry （FCM）. Results Both ELISA and indirect immunofluorescence proved the binding of natural lgM 3B4 to S. autreus. Colony forming assay found that the amount of colony forming units decreased significantly when 3B4 was added. The analysis of FCM showed that 3B4 augmented phagocytosis of S, aureus hy phagocytes. Conclusions Natural antikeratin IgM 3B4 can bind to S. aureus and regulate the phagocytosis of it, indicating that natural lgM may play some role in the defense against bacterial infection.

关 键 词：免疫球蛋白M 乔噬作用 葡萄球菌 金黄色 金黄色葡萄球菌 天然抗角蛋白 巨噬细胞吞噬 IGM抗体 调理 间接免疫荧光法 

分 类 号：R392.1[医药卫生—免疫学]