Tcl/Tc2失衡和慢性光线性皮炎发病的相关性研究  

Imbalance of Tc Cells in the Pathogenesis of Chronic Actinic Dermatitis

作　　者：陈浩[1] 邓丹琪[1] 周晓鸿[1] 樊应俊[1] 杨红英[2] 

机构地区：[1]昆明医学院第二附属医院皮肤性病科,650101 [2]昆明医学院第二附属医院检验科,650101

出　　处：《中华皮肤科杂志》2005年第10期628-630,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金(30260094)

摘　　要：目的探讨T_c细胞失衡和慢性光线性皮炎(CAD)发病的相关性。方法应用免疫组化SP法检测28例CAD患者和7例正常人对照者皮肤中真皮浸润细胞的免疫表型及白介素4、10、12,干扰素γ受体的表达。同时应用ELISA的方法检测33例CAD和32例正常人对照者血清中白介素4、10、12、干扰素γ的含量。结果 CAD患者皮损真皮内浸润的单一核细胞绝大部分表达为CD3^+阳性的T细胞,阳性细胞百分数,平均灰度与CD20^+阳性的B细胞相比有统计学意义(P均＜0．001)。这些T细胞中大部分表达为CD8^+阳性的T细胞,阳性细胞百分数和平均灰度与CD4^+阳性的T细胞相比有统计学意义(P均＜0．001)。Tcl细胞分泌干扰素γ,白介素12的受体在CAD患者真皮内浸润的Tc细胞表面的表达要高于Tc_2细胞分泌的白介素10、4的受体表达(P均＜0．001)。外周血干扰素γ、白介素12含量在CAD患者组和对照组中有统计学意义(P均＜0．05)。结论 CAD发展可能和Tcl细胞的过度表达及Tc2细胞的表达受抑有一定的关系。Objective To investigate the association of imbalance of Tcl and Tc2 cells with chronic actinic dermatitis （CAD）. Methods lmmunohistochemistry （S-P method） was used to test the subgroups of infiltrating cells and the expression of reeeptors of interleukin-4 （IL-4）, interleukin-10（IL-10）, interleukin- 12 （IL-12） and interferon-γ （IFN-γ） in these cells of the eruptions from 28 patients with CAD and 7 normal controls. The serum levels of IL-4, II.-lO, IL-12 and IFN-γ from 33 patients with CAD and 32 normal controls were tested by ELISA. Results The major infiltrating cells in the eruptions of patients with CAD were T cells, which were significantly more than B cells （P 〈 0.001 ）. Among these T cells, the predominant was Tc cells, which were significantly more than Th cells （P 〈 0.001 ）. In the case of infiltrating cells, the expression of receptors of IL-12 and IFN-γ, was significantly higher than that of receptors of IL-4 and IL-10 （P 〈 0.001 ）. The serum levels of IL-12 and IFN-γ in patients with CAD were significantly increased than those in the control group （both P 〈 0.05 ）. Conclusions These findings indicate the imbalance of Tcl and Tc2 cells probably plays an important role in the etiology and pathogenesis of CAD.

关 键 词：光过敏疾患 T淋巴细胞 辅助诱导 T淋巴细胞 细胞毒性 白细胞介素类 干扰素Ⅱ型 TC1/TC2 细胞失衡 光线性 

分 类 号：R758.1[医药卫生—皮肤病学与性病学]