大鼠实验性胃溃疡自愈期间肠嗜铬样细胞的变化  

Change in enterochromaffin-like cells during healing of experimental gastric ulcer in rats

作　　者：何美蓉[1] 张绍荣[1] 宋于刚[1] 陈学清[1] 

机构地区：[1]南方医科大学南方医院消化内科实验室,广东广州510515

出　　处：《第四军医大学学报》2005年第19期1735-1737,共3页Journal of the Fourth Military Medical University

基　　金：广东省自然科学基金(010578)

摘　　要：目的:探讨肠嗜铬样细胞与大鼠实验性胃溃疡自愈的关系.方法:通过制备大鼠乙酸性胃体溃疡模型,采用免疫组化、逆转录聚合酶链反应(RT-PCR)和放射免疫测定的方法,观察溃疡自愈过程中大鼠胃黏膜内ECL细胞形态、组氨酸脱羧酶(HDC)mRNA和组胺含量的变化.结果:胃溃疡大鼠胃黏膜内HDC阳性细胞率和胞质平均灰度值在制模术后第1日即开始降低,术后第6日达到最低,随后开始回升,术后第12日基本正常.胃黏膜内HDC mRNA表达在制模术后第1日开始降低,第3日最明显,第6日开始恢复,第9日后接近正常.制模术后胃黏膜内组胺含量也出现下调,以术后第6日为最低.结论:ECL细胞可通过减弱其合成组胺的功能。AIM. To investigate the morphologic and functional changes in enterochromaffin-like （ECL） cells during the healing of experimental gastric ulcer in rats. METHODS： Acetic acid-induced ulcers were caused by application of acetic acid to the serosal surface of the anterior face of the rat gastric bodies. At different time points during ulcer healing, ECL cells, histidine decarboxylase （HDC） mRNA expression and histamine content were studied using immunohistochemical staining, reverse transcription-polymerase chain reaction and radioimmunoassay, respectively. RESULTS： Compared with that in control group, the percentage and the mean gray value of HDC-positive cells in gastric mucosa of ulcer rats decreased immediately on day 1, most markedly on day 6. They began to increase on day 9 and returned to the initial levels on day 12. The HDC mRNA expression in gastric mucosa of ulcer rats decreased on day 1, most markedly on day 6, subsequently rose gradually and almost normalized on day 9. The histamine content in gastric mucosa of ulcer rats also decreased, lowest on day 6 after ulcer induction. CONCLUSION： ECL cells maybe participate in the regulation of ulcer healing by weakening the function of histamine synthesis.

关 键 词：胃溃疡 肠嗜铬样细胞 组氨酸脱羧酶 组胺 

分 类 号：R322.5[医药卫生—人体解剖和组织胚胎学]