脑源性神经营养因子转基因细胞移植和甲基强的松龙对损伤脊髓caspase-3表达的影响  

Effect of intraspinal grafting of adenovirus-mediated brain derived neurotrophic factor ex vivo transgene myoblasts cells and methylprednisolone on expression of caspase-3 after spinal cord injury

作　　者：张强[1] 邹德威[1] 海涌[1] 马华松[1] 王拥军[2] 

机构地区：[1]解放军第三○六医院骨科,北京100101 [2]上海中医药大学脊柱病研究所

出　　处：《中华创伤杂志》2005年第10期733-736,共4页Chinese Journal of Trauma

摘　　要：目的探讨大鼠脊髓损伤后腺病毒介导的脑源性神经营养因子(AxCA-BDNF)体外转基因成肌细胞移植和静脉内注射大剂量甲基强的松龙(MP)对大鼠脊髓细胞caspase-3表达的影响。方法将120只大鼠分为脊髓挫伤组(A组)、脊髓挫伤后AxCA-BDNF基因转染的成肌细胞移植组(B组)、脊髓挫伤后静脉内注射大剂量甲基强的松龙治疗组(C组)、AxCA-BDNF基因转染的成肌细胞移植和静脉内注射大剂量甲基强的松龙治疗组(D组)。伤后1,3,7,14,28d,应用免疫组化法对脊髓损伤区进行细胞凋亡caspase-3蛋白表达的测定,并采用计算机图像分析技术进行定量分析。应用行为学和电生理检查方法观察大鼠运动功能恢复情况。结果A、B、C、D四组中均发现凋亡caspase-3蛋白阳性表达细胞;各组caspase-3免疫反应阳性细胞数依次为A>B>C>D组(P<0.05),与大鼠后肢功能恢复有平行的变化趋势。结论体外转基因成肌细胞移植和大剂量甲基强的松龙能抑制脊髓损伤后的细胞凋亡。Objective To investigate the eiiect ot intraspinal gralting ot brain derived neurotrophic factor （BDNF） ex vivo transgene myoblasts cells and methylprednisolone on caspase-3 expression after spinal cord injury （SCI）. Methods A total of 120 experimental rats were divided into Group A （spinal cord contusion injury group）, Group B （grafting of BDNF ex vivo transgene myoblasts ceils group） , Group C （methylprednisolone intravenous injection group） and Group D （grafting of BDNF ex vivo transgene myoblasts cells and methylprednisolone intravenous injection group）. At days 1, 3, 7, 14 and 28 respectively after SCI, the expression of caspase-3 was measured immunohistochemically for quantitative analysis via a computer image analysis system. The motion functional recovery of the rats was observed by praxiologic and electrophysiologic examination. Results Positive expression cells of caspase- 3 were found in all groups, with number from the highest to the lowest in order of Group A, Group B, Group C and Group D （ P 〈 0.05 ） , which was parallel to improvement trend of neurological function recovery of the posterior extremity. Conclusion The intraspinal grafting of BDNF ex vivo transgene myoblasts ceils and high dose of methylprednisolone can inhibit the cell apoptosis following SCI.

关 键 词：脊髓损伤 转基因 脑源性神经营养因子 CASPASE类 甲基强的松龙 CASPASE-3表达 大剂量甲基强的松龙 转基因细胞移植 损伤脊髓 CASPASE-3蛋白 

分 类 号：R651.2[医药卫生—外科学]