RDP1258免疫调节功能在大鼠体内实验研究  

Study of the immunomodulatory effect of RDP1258 in vivo

作　　者：易善红[1] 宋波[2] 叶钢[1] 汪泽厚[3] 

机构地区：[1]第三军医大学新桥医院泌尿外科,全军肾脏病中心重庆400037 [2]第三军医大学西南医院全军泌尿外科中心,重庆400038 [3]第三军医大学解放军空军总医院泌尿外科,北京100036

出　　处：《第三军医大学学报》2005年第17期1748-1751,共4页Journal of Third Military Medical University

基　　金：国家新药研究基金资助项目(9690105184)~~

摘　　要：目的观察一种新型HLA衍生肽(RDP1258)的体内免疫抑制功能并探讨其机制。方法人工固相合成HLA衍生肽(RDP1258),以3H-TdR法观察其在体内对大鼠脾细胞增殖反应的影响;与HO-1酶活性激动剂HEM IN及拮抗剂ZNPP相对比,采用酶化学法及免疫组化方法观察其对体内脾细胞血红素氧合酶-1(hem e oxygenase-1,HO-1)活性的影响。结果RDP1258体内应用可显著抑制淋巴细胞转化及MLR的增殖反应;该肽能够明显提高体内HO-1活性,并增强HO-1的表达。结论RDP1258体内应用能够显著抑制大鼠脾细胞由丝裂原或同种抗原引起的增殖反应,这种作用可能是通过影响HO-1活性而达到的。Objective To observe the immunosuppressive function of a novel HLA-derived peptide,RDP1258, and to investigate the mechanisms in vivo. Methods A peptide derived from HLA, RDP1258, was chemically synthesized. The effects of the peptide on alloreactive cytotoxic activities of rat spleen cells in vivo were observed using ZH-TDR method. As compared with the HO-1 inducer hemin and the HO-1 inhibitor zinc protoporphyrin （ZnPP）, the HO enzyme activity was analyzed by enzyme chemistry method. Results The in vivo administration of the synthetic HLA-derived peptide can obviously inhibit the proliferation of rat spleen cells stimulated by isoantigen and mitogen, and resulted in upregulation of splenic heme oxygenase activity and rapid upregulation of HO-1 protein expression. Conclusion RDP1258 inhibits the proliferation of rat spleen cells induced by mitogen and isoantigen probably through enhancing the HO-1 activity.

关 键 词：RDP1258 HLA衍生肽 混合淋巴细胞反应 血红素氧化酶-1 

分 类 号：Q516[生物学—生物化学] R392.1[医药卫生—免疫学]