Bcl-2蛋白、基因在Parkinson病鼠黑质内的表达及中药对其表达的影响  

PROTEIN AND mRNA LEVELS FOR Bcl-2 IN THE SUBSTANTIA NIGRA OF PARKINSON'S DISEASE(PD) MICE AND THE EFFECT OF CHINESE MEDICINE ON THEM

作　　者：孙红梅[1] 白丽敏[1] 张军[1] 许红[1] 吴海霞[1] 崔龙[1] 

机构地区：[1]北京中医药大学解剖学教研室,北京100029

出　　处：《神经解剖学杂志》2005年第5期507-510,共4页Chinese Journal of Neuroanatomy

基　　金：清华大学资助项目;香港浸会大学资助项目;北京中医药大学"中医研究计划"资助项目

摘　　要：采用MPTP腹腔注射建立Parkinson病动物模型。选用健康C57BL/6J小鼠随机分成3组:正常组、模型组和中药治疗组。动物存活期分别为14d和28d。采用ABC免疫组化和原位杂交方法对各组脑切片分别进行Bcl-2蛋白和mRNA检测。结果表明:模型组Parkinson病鼠中脑黑质内Bcl-2及其mRNA的表达在两个时段均明显低于正常组。其光密度28d比14d降低更为明显,而中药可部分上调Bcl-2及其mRNA的表达。提示:Bcl-2可能参与了Parkinson病中黑质多巴胺神经元的凋亡过程,中药可通过调节凋亡相关蛋白和基因的表达,阻止多巴胺神经元的凋亡,从而达到防治Parkinson病的目的。C57BL/6J mice were injected intraperitoneally with MPTP to induce Parkinson＇s disease model. The experimental animals were divided randomly into three groups：（1）The normal control group （NC）;（2）The model control group（MC）;（3） Chinese medicine group（CM）;The animals were killed respectively on the 14^th or the 28^th day after first injection of MPTP. ABC immunohistochemistry and in situ hybridization techniques were used to detect the expression for Bcl-2 protein or mRNA. respectively. The results showed that the expressions for Bcl-2 and bcl-2 mRNA were less than those of normal control in substantia nigra（SN） of Parkinson＇s disease mice. The expressions were less on the 28^th day than those of the 14^th day. Reactivity for Bcl-2 and bcl-2 mRAN increased in SN of CM animals. The results suggest that bcl-2 may play an important role in apoptosis of dopaminergic neurons in PD.Chinese medicine may attenuate apoptosis of dopaminergic neurous by up-regulating the expression for Bcl-2 and bcl-2 mRAN.

关 键 词：BCL-2 中约 黑质 Parkinson病鼠 BCL-2蛋白 中药治疗 小鼠 基因 多巴胺神经元 mRNA 

分 类 号：R285[医药卫生—中药学] R361[医药卫生—中医学]